A mixed light field generated from a two-level atomic ensemble can be used for two-photon interference. In this mixed light field, correlated paired photons generated from a four-wave mixing process provide a signal of two-photon interference, while Rayleigh scattered photons of the pump laser provide a stable reference to calibrate the normalized second-order correlation function. We demonstrate two-photon interference using the Hong–Ou–Mandel and Hanbury Brown–Twiss interferometers. A direct quantitative comparison between theoretical predictions and experimental data is performed under perturbed experimental conditions, which reveal this kind of light source has potential application for quantum metrology.