针对微血管中血流速度慢、血红细胞数量少的特点,采用同步移相显微干涉术实时测量血红细胞的相位,建立血红细胞特征体积模型,对多帧相位图进行特征血红细胞匹配,以实现慢速血流检测。利用牛红细胞阿氏液制备了流速在0.1~1.0 mm/s内可控,内径为100 μm的微血管模型,搭建了基于微偏振阵列的同步移相显微干涉实验装置,通过实验验证了所提慢速血流检测方法的可行性,血流速度的测量误差不超过±11.2%。

Simultaneous phase-shifting interferometric microscopy is proposed herein to measure the phase of red blood cells for leveraging the characteristics of slow blood flow and a small number of red blood cells in microvessels. A characteristic volume model of red blood cells is established to match characteristic red blood cells from multi-frame phase maps. Then, slow blood flow detection is realized. A Ф100-μm microvessel prototype with a flow rate of 0.1-1.0 mm/s is prepared using Alsever’s solution of bovine red blood cell. A simultaneous phase-shifting interferometric microscopy experimental setup based on a micro-polarization array is constructed to validate the feasibility of the proposed detection method, and the measurement error of the blood flow velocity is no greater than ±11.2%.