中国光学期刊网

Photoacoustic imaging has been developed to image the immune study at the macro scale. Macrophages play diverse roles in the acute response to infection and tissue repair. However, macrophages activities in acute inflammation at the microscopic level still remain challenging. In this work, we proposed optical-resolution photoacoustic microscopy to promptly monitor the labeled macrophages activities in normal and inflammatory groups. The result showed that many labeled macrophages emerged around the vessels firstly, then exuded into tissues, and finally disappeared in the inflammatory group injected with labeled macrophages. In summary, our method allows us to exactly image and track the immune cells of inflammatory diseases.

用He-Ne激光辐照巨噬细胞,实时观测活体单细胞内钙浓度([Ca2+]i)分布和细胞免疫活性随激光功率和照射时间的变化.实验结果表明,随激光照射时间的增加,[Ca2+]i出现上升达到最大值后又下降,逐渐回复初始状态,且呈现中心最强,径向衰减的环形梯度分布.不同激光剂量对巨噬细胞内[Ca2+]i及其免疫活性有着不同的影响,同一波长激光,当剂量不同时,可以表现为完全相反的效应.同时激光功率也是影响巨噬细胞内[Ca2+]i及其免疫活性的一个重要因素,同一波长、同一剂量情况下,如果激光功率不同,巨噬细胞内[Ca2+]i及其免疫活性的变化也有着显著的差异.当照射功率为0.16 mW时,胞内[Ca2+]i峰值是照射功率为0.40 mW时的近6倍.最大值时对应细胞免疫活性最高.对其机理作了初步探讨.