本文研究了内窥式散斑类共聚焦系统(Endoscope-based speckle quasi-confocal system, EBSQCS)的层析能力。从散斑类共聚焦显微镜(Speckle quasi-confocal microscope, SQCM)成像原理出发,详细分析了内窥镜光学结构对散斑类共聚焦显微镜散斑场波动的影响规律,推导了内窥镜光学结构与内窥式散斑类共聚焦系统轴向分辨力的关系。实验测得了基于光纤束的内窥式散斑类共聚焦系统的轴向分辨力曲线。选用放大倍率4倍,光纤直径5 μm的内窥镜系统的内窥式散斑类共聚焦系统轴向分辨力曲线的全峰半高是散斑类共聚焦显微镜的2.3倍,与理论计算值相符。实验结果表明内窥式散斑类共聚焦系统具有很好的轴向层析能力。

The sectioning capability of the endoscope-based speckle quasi-confocal system (EBSQCS) is studied. Based on the theory of speckle quasi-confocal microscope (SQCM), the influence of endoscope on the discipline of speckle quasi-confocal microscope speckle fluctuation is discussed, and analytical expression is derived to describe the relationship between optical structure of endoscope and the axial resolution of Endoscope-based speckle quasi-confocal system. The axial resolution curve is also obtained by experiment of Endoscope-based speckle quasi-confocal system with a fiber bundle. The full width at half maximum (FWHM) of Endoscope-based speckle quasi-confocal system resolution curve is 2.3 times of that of speckle quasi-confocal microscope when the endoscope has a magnification of 4 times and fiber diameter of 5 μm, which is in accordance with the theory. The experimental result shows that eEndoscope-based speckle quasi-confocal system has the quality of axial optical sectioning.