光学学报, 2007, 27 (7): 1245, 网络出版: 2007-08-17   

内窥式散斑类共聚焦系统层析能力分析

Sectioning Capability of the Endoscope-Based Speckle Quasi-Confocal System
作者单位
华中科技大学生物医学光子学教育部重点实验室, 武汉 430074
摘要
本文研究了内窥式散斑类共聚焦系统(Endoscope-based speckle quasi-confocal system, EBSQCS)的层析能力。从散斑类共聚焦显微镜(Speckle quasi-confocal microscope, SQCM)成像原理出发,详细分析了内窥镜光学结构对散斑类共聚焦显微镜散斑场波动的影响规律,推导了内窥镜光学结构与内窥式散斑类共聚焦系统轴向分辨力的关系。实验测得了基于光纤束的内窥式散斑类共聚焦系统的轴向分辨力曲线。选用放大倍率4倍,光纤直径5 μm的内窥镜系统的内窥式散斑类共聚焦系统轴向分辨力曲线的全峰半高是散斑类共聚焦显微镜的2.3倍,与理论计算值相符。实验结果表明内窥式散斑类共聚焦系统具有很好的轴向层析能力。
Abstract
The sectioning capability of the endoscope-based speckle quasi-confocal system (EBSQCS) is studied. Based on the theory of speckle quasi-confocal microscope (SQCM), the influence of endoscope on the discipline of speckle quasi-confocal microscope speckle fluctuation is discussed, and analytical expression is derived to describe the relationship between optical structure of endoscope and the axial resolution of Endoscope-based speckle quasi-confocal system. The axial resolution curve is also obtained by experiment of Endoscope-based speckle quasi-confocal system with a fiber bundle. The full width at half maximum (FWHM) of Endoscope-based speckle quasi-confocal system resolution curve is 2.3 times of that of speckle quasi-confocal microscope when the endoscope has a magnification of 4 times and fiber diameter of 5 μm, which is in accordance with the theory. The experimental result shows that eEndoscope-based speckle quasi-confocal system has the quality of axial optical sectioning.
参考文献

[1] . Wilson, R. Juskaitis, M. A. A. Neil et al.. Confocal microscopy by aperture correlation[J]. Opt. Lett., 1996, 21(23): 1879-1881.

[2] . A. A. Neil, R. Juskaitis, T. Wilson. Method of obtaining optical sectioning by using structured light in a conventional microscope[J]. Opt. Lett., 1997, 22(24): 1905-1907.

[3] . Quasi-confocal fluorescence sectioning with dynamic speckle illumination[J]. Opt. Lett., 2005, 30(24): 3350-3352.

[4] . Dynamic speckle illumination microscopy with translated versus randomized speckle patterns[J]. Opt. Exp., 2006, 14(16): 7198-7209.

[5] Ge Huayong, Ren Qiushi, Wang Baohua et al.. Confocal endoscopic optical imaging system[J]. Laser & Optoelectronics Progress, 2004, 41(8): 32~34 (in Chinese)
葛华勇,任秋实,王保华 等. 共聚焦内窥镜光学成像系统[J]. 激光与光电子学进展, 2004, 41(8): 32~34

[6] Jia Xiaohang, Yan Qinglai, Wen Yan. Evaluation base and method of medical rigid endoscope distortion[J]. Acta Optica Sinica, 2006, 26(8): 1226~1230
贾晓航,颜青来,文燕. 医用硬性内窥镜畸变的评定基础和方法[J]. 光学学报, 2006, 26(8): 1226~1230

[7] Fang Fang, Lin Meirong, Li Yingjie et al.. Design and study of micro-fiber imaging system[J]. Acta Optica Sinica, 2000, 20(5): 654~658
房芳,林美荣,李英杰 等. 微型光纤图像传输系统的设计与研究[J]. 光学学报, 2000, 20(5): 654~658

[8] . Gmitro, David Aziz. Confocal microscopy through a fiber-optic imaging bundle[J]. Opt. Lett., 1993, 18(8): 565-567.

[9] Andrew R. Rouse, Angeligue Kano, Arthur F. Gmitro. Development of a fiber-optic confocal microendoscope for clinical endoscopy[C]. SPIE, 2002, 4613: 244~253

[10] Cathie Ventalon, Jerome Mertz. Quasi-confocal fluorescence sectioning with dynamic speckle illumination[C]. SPIE, 2006, 6091: 60900M-1~60900M-6

[11] . D. Kerr, Axel Nimmerjahn et al.. Miniaturized two-photon microscope based on a flexible coherent fiber bundle and a gradient-index lens objective[J]. Opt. Lett., 2004, 29(21): 2521-2523.

[12] Liu Cihua, Wan Jianping. Probability and Mathematical Statistics[M]. Beijing: China Higher Education Press, 1999. 113~119 (in Chinese)
刘次华,万建平. 概率论与数理统计[M]. 北京: 中国高等教育出版社,1999. 113~119

吴萍, 吕晓华, 易秋实, 骆清铭, 曾绍群. 内窥式散斑类共聚焦系统层析能力分析[J]. 光学学报, 2007, 27(7): 1245. 吴萍, 吕晓华, 易秋实, 骆清铭, 曾绍群. Sectioning Capability of the Endoscope-Based Speckle Quasi-Confocal System[J]. Acta Optica Sinica, 2007, 27(7): 1245.

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