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Nonlinear spectral imaging microscopy of rabbit aortic wall

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Abstract

Employing nonlinear spectral imaging technique based on two-photon-excited fluorescence and second-harmonic generation (SHG) of biological tissue, we combine the image-guided spectral analysis method and multi-channel subsequent detection imaging to map and visualize the intrinsic species in a native rabbit aortic wall. A series of recorded nonlinear spectral images excited by a broad range of laser wavelengths (730-910 nm) are used to identify five components in the native rabbit aortic wall, including nicotinamide adenine dinucleotide (NADH), elastic fiber, flavin, porphyrin derivatives, and collagen. Integrating multi-channel subsequent detection imaging technique, the high-resolution, high contrast images of collagen and elastic fiber in the aortic wall are obtained. Our results demonstrate that this method can yield complementary biochemical and morphological information about aortic tissues, which have the potential to determine the tissue pathology associated with mechanical properties of aortic wall and to evaluate the pharmacodynamical studies of vessels.

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收稿日期:2008-09-08

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网络出版日期:0001-01-01

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刘全纲:Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007
陈建新:Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007
卓双木:Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007
姜兴山:Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007
卢可成:Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou 350007

备注:This work was supported by the National Natural Science Foundation of China (No.60508017), the Natural Science Foundation of Fujian Province (No.2007J0007, C0720001), the Science and Technology Planning Key Program of Fujian Province (No.2008Y0037), and the Program for New Century Excellent Talents in University of China (NCET-07-0191). E-mail: chenjianxin@fjnu.edu.cn

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引用该论文

Quangang Liu, Jianxin Chen, Shuangmu Zhuo, Xingshan Jiang, Kecheng Lu, "Nonlinear spectral imaging microscopy of rabbit aortic wall," Chinese Optics Letters 7(3), 240-243 (2009)

被引情况

【1】李进峰,梁晓燕,何晋平,林华. 稳定高效的半导体泵浦Yb:KGW飞秒激光器. Chinese Optics Letters, 2011, 9(7): 71406-71405

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