抑制血管生成是肿瘤治疗的重要方法之一,若能从分子水平检测血管损伤的机制,对治疗将更具指导意义。拉曼光谱是一种无损、信息非常丰富的光谱技术,可以应用于固态、溶液或液态的生物分子分析。基于激光共焦拉曼光谱技术设计一方案,测量分析了鸡胚微血管在抗血管药物沙利度胺作用前后的拉曼光谱。加药5 h与对照组血管的平均拉曼谱在位置和形状上类似,但某些特征峰在强度上却发生变化,如波数1441 cm-1,1527 cm-1及1657 cm-1的强度明显增强而971 cm-1和1081 cm-1的强度明显减弱,这些变化可能是药物作用血管后,使血管内的核酸、蛋白质、磷脂等重要生物分子在结构或含量上发生了不同的改变而引起的。研究结果表明拉曼光谱有可能成为从分子水平分析抗血管生成药物对血管作用的一种有效方法。

Inhibit angiogenesis is one of important tumor therapies. If the mechanism of vascular changes can be detected from molecular lever,it will have therapeutic significance. Raman spectroscopy is a non-destructive spectrum technology holding rich information,which can be applied to the structural analysis of solid,liquid or solution of biological molecules. Based on confocal Raman microscope,a unique system is developed for obtaining the different Raman spectrum about vessels of the chick embryo with and without the anti-angiogenic drugs-thalidomide. In the study,the location and shape of the average Raman spectrum of vessels in drug 5 h is similar to the ones without medicine,but the intensity of some characteristic peaks changes. Intensity at 1441 cm-1,1527 cm-1and 1657 cm-1,increases markedly,while the 971 cm-1 and 1081 cm-1 decreases. The change is due to vascular medicine which causes the nucleic acid,protein,phospholipids and other important biological molecules in the vessels to change on the structure or content.The result indicates that Raman spectroscopy could be an effective tool for detection of the mechanism of vascular changes.