Chinese Optics Letters, 2010, 8 (2): 236, Published Online: Mar. 5, 2010
Study on interaction between Dy(III)(NR)3 complex and herring sperm DNA by spectroscopy 
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Dy(Ⅲ)(NR)3配合物 鲱鱼精DNA 吖啶橙 作用模式 300.6390 Spectroscopy, molecular 300.6280 Spectroscopy, fluorescence and luminescence 300.6550 Spectroscopy, visible
Abstract
The action mode between Dy(III)(NR)<sub>3</sub> and herring sperm DNA is studied by ultraviolet-visible (UV-vis) and fluorescence spectra as well as electrochemistry. Double-reciprocal method studies show that the binding stoichiometry between Dy(III)(NR)<sub>3</sub> and DNA is 1:1, the binding constants at different temperatures are 1。15×10<sup>5</sup> L/mol at 25 ℃ and 2.09×10<sup>5</sup> L/mol at 35 ℃, and the corresponding thermodynamic parameters are \Delta<sub>r</sub>H \mathrm m = 2.48×10<sup>4</sup> J/mol, \Delta<sub>r</sub>G \mathrm m =-2.34×10<sup>4</sup> J/mol, \Delta<sub>r</sub>Sm=161.7 J/(mol·K); \Delta<sub>r</sub>S \mathrm m is the driving force in this reaction. Combined with Scatchard method and melting method, the results suggest that the interaction mode between Dy(III)(NR)<sub>3</sub> and herring sperm DNA is intercalation fashion and groove fashion.
Yan Zhang, Xingming Wang, Dan Fei, Na Zhao, Tingting Zhao, Haifeng Zhu, Lisheng Ding. Study on interaction between Dy(III)(NR)3 complex and herring sperm DNA by spectroscopy[J]. Chinese Optics Letters, 2010, 8(2): 236.
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