Chinese Optics Letters, 2010, 8 (10): 944, Published Online: Oct. 19, 2010
A miniature laser speckle fluorescence sectioning microscope for cell imaging Download: 662次
激光散斑 荧光层析 细胞成像 170.1790 Confocal microscopy 180.1790 Confocal microscopy 180.2520 Fluorescence microscopy 180.6900 Three-dimensional microscopy
Abstract
We present a miniature fluorescence sectioning microscope which uses a diode-pumped solid-state (DPSS) laser as the light source and a fast translating diffuser to produce dynamically changing speckle patterns onto the back aperture of the objective to illuminate the sample. Optical sectioning, which originates from the statistical characteristics of laser speckles, is obtained by calculating the contrast of a series of fluorescence images. High contrast fluorescence sectioning images of bovine pulmonary artery endothelial (BPAE) cells are obtained. The image quality is similar to that of the images acquired by standard laser scanning confocal microscope (LSCM). Compared with LSCM, the laser speckle fluorescence microscope (LSFM) presented in this letter has many advantages, such as simple configurations, low cost, compact, and easy to operate, which makes it possible to have wide spread applications in biomedicine.
Yonghong Shao, Heng Li, Qiao Wen, Yan Wang, Junle Qu, Hanben Niu. A miniature laser speckle fluorescence sectioning microscope for cell imaging[J]. Chinese Optics Letters, 2010, 8(10): 944.