发光学报, 2012, 33 (9): 1030, 网络出版: 2012-09-24
基于FRET机理的荧光探针的合成及其对汞离子的识别
Synthesis of Fluorescent Probe Based on FRET and Its Appalication in The Determination for Hg2+
摘要
基于FRET机理设计合成了一个包含罗丹明6G及香豆素的汞离子荧光探针Rh-6G-coumarin(RC)，研究了它的光谱性能及对汞离子的识别作用。在V(C2H5OH)∶V(H2O)=9∶1溶液中加入汞离子后，575 nm处荧光强度迅速增大，荧光由蓝色变为明亮的红色，同时溶液的颜色由黄色变为红色。溶液中其他金属离子，如Na+、K+、Mg2+、Fe2+、Co2+、Pb2+、Zn2+、Cd2+和Cr3+对汞离子的荧光识别没有太大影响。该探针可在较宽的pH ( 4～10)范围内识别汞离子。光谱滴定实验表明，汞离子与RC以2∶1的计量比形成了配合物。
Abstract
By combining rhodamine 6G and cumarin, a fluorescent probe of Hg2+, Rh-6G-cumarin(RC), based on FRET mechanism was synthesized. And through research its spectral properties and recognition to mercury ion, we found that it featured a rapid fluorescence enhancement at 575 nm upon the addition of Hg2+ in V(C2H5OH)∶V(H2O)=9∶1 solution. The fluorescence changed from blue to bright red, while the color of the solution changed from yellow to red. This probe showed high specific for Hg2+ over other metal cations, such as Na+,K+, Mg2+, Cr3+, Fe2+,Co2+,Zn2+, Cd2+ and Pb2+. It could detect Hg2+ effectively in a relatively wide pH range (4～10). The titration experiment indicated a 2∶1 stoichiometry of the RC-Hg2+ complexation species.
王海菊, 张文珠, 何成, 周硼. 基于FRET机理的荧光探针的合成及其对汞离子的识别[J]. 发光学报, 2012, 33(9): 1030. WANG Hai-ju, ZHANG Wen-zhu, HE Cheng, ZHOU Peng. Synthesis of Fluorescent Probe Based on FRET and Its Appalication in The Determination for Hg2+[J]. Chinese Journal of Luminescence, 2012, 33(9): 1030.
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