Chinese Optics Letters, 2013, 11 (1): 011703, Published Online: Jan. 29, 2013
Recent advances in two-photon imaging: technology developments and biomedical applications Download: 1007次
170.3880 Medical and biological imaging 170.0170 Medical optics and biotechnology 180.4315 Nonlinear microscopy 180.0180 Microscopy 170.2150 Endoscopic imaging 170.0170 Medical optics and biotechnology 190.4180 Multiphoton processes 190.0190 Nonlinear optics 170.6510 Spectroscopy, tissue diagnostics 170.0170 Medical optics and biotechnology
Abstract
In the past two decades, two-photon microscopy (TPM) transforms biomedical research, allowing nondestructive high-resolution fluorescent molecular imaging and label-free imaging in vivo and in real time. Here we review the recent advances of TPM technology including novel laser sources, new image acquisition paradigms, and microendoscopic imaging systems. Then, we survey the capabilities of TPM imaging of biological relevant molecules such as nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD), and reactive oxygen species (ROS). Biomedical applications of TPM in neuroscience and cancer detection are demonstrated.
Yu Chen, Hengchang Guo, Wei Gong, Luye Qin, Hossein Aleyasin, Rajiv R, Sunghee Cho, Jianxin Chen, Shusen Xie. Recent advances in two-photon imaging: technology developments and biomedical applications[J]. Chinese Optics Letters, 2013, 11(1): 011703.