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Recent advances in two-photon imaging: technology developments and biomedical applications

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Abstract

In the past two decades, two-photon microscopy (TPM) transforms biomedical research, allowing nondestructive high-resolution fluorescent molecular imaging and label-free imaging in vivo and in real time. Here we review the recent advances of TPM technology including novel laser sources, new image acquisition paradigms, and microendoscopic imaging systems. Then, we survey the capabilities of TPM imaging of biological relevant molecules such as nicotinamide adenine dinucleotide (NADH), flavin adenine dinucleotide (FAD), and reactive oxygen species (ROS). Biomedical applications of TPM in neuroscience and cancer detection are demonstrated.

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DOI:10.3788/col201311.011703

所属栏目:Medical optics and biotechnology

责任编辑:刘盛龄

收稿日期:2012-10-30

录用日期:2012-12-07

网络出版日期:2013-01-06

联系人作者:联系作者(ssxie@fjnu.edu.cn)

备注:supported by National Institutes of Health (NIH) under Grant Nos. R21AG042700, R21DK088066, and R21EB012215. L. Qin and S. Cho are supported by NIH under Grant No. R01HL082511. H. Aleyasin and R. R. Ratan are supported by NIH under Grant Nos. P01 AG 14930-10, NS04059, and NS39170. W. Gong, J. Chen, and S. Xie are supported by the National Natural Science Foundation of China under Grant No. 60678054.

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引用该论文

Yu Chen, Hengchang Guo, Wei Gong, Luye Qin, Hossein Aleyasin, Rajiv R Ratan, Sunghee Cho, Jianxin Chen, Shusen Xie, "Recent advances in two-photon imaging: technology developments and biomedical applications," Chinese Optics Letters 11(1), 011703 (2013)

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