基线漂移是目前光谱仪收集拉曼光谱时难以避免的现象, 基线校正是光谱数据处理中一个不可或缺的重要步骤。 针对传统的基于多项式拟合的基线校正方法存在的不足, 本工作采用线性拟合的方法分段拟合背景基线, 与计算机相结合, 实现快速、 准确、 自动的基线校正; 经聚苯乙烯小球、 红细胞和酿酒酵母等多批次拉曼光谱数据处理的验证, 表明该改进方法可以有效地对拉曼光谱进行基线校正, 为进一步分析光谱数据提供更准确的信息, 是一种可行的基线校正方法。

The baseline drifts of Raman spectra occur in many types of instrumental measurements. It is an important part and a routine step to correct the baseline drift for the data preprocessing. In the present work, the limitations of the baseline correction method based on polynomial fitting were highlighted and a modified polynomial fitting method, i.e. piecewise linear fitting method, was proposed. Combined with the computer, this method could eliminate the baseline automatically. A series of Raman spectra of single polystyrene bead, red blood cell or yeast cell acquired by laser tweezers Raman spectroscopy were preprocessed by this method and its efficiency was verified. The results demonstrated that piecewise linear fitting can correct the baseline shifts effectively and provides more accurate information for further data analysis. It is a feasible method for correction of Raman spectrum.