研究激光针灸在不同的作用方式和参数设置下，对Ⅱ型胶原蛋白诱导的关节炎(CIA)模型大鼠血清白细胞介素1β（IL-1β）、白细胞介素15（IL-15）、白细胞介素17（IL-17）、肿瘤坏死因子α（TNF-α）、血管内皮细胞生长因子（VEGF）和皮质醇（COR）的影响。将大鼠随机分为模型组，药物组，针刺组，深部激光针灸1 mW组、5 mW组、10 mW组，表面激光针灸50 mW组、100 mW组、150 mW组，并设正常对照组，针灸穴位选择足三里和肾俞。治疗10 d后取材，采用双抗体夹心酶联免疫吸附试验法（ELISA）测定血清因子水平。模型组的血清IL-1β、IL-15、IL-17、TNF-α和VEGF均较正常组显著升高，有统计学意义（均为P＜0.001），COR较正常组显著降低，有统计学意义（P＜0.001）。治疗后，各治疗组的IL-1β、IL-15、IL-17、TNF-α和VEGF均较模型组有不同程度的降低，除个别组外，与模型组的差异均有统计学意义（P＜0.05、P＜0.01、P＜0.001）；各治疗组的COR均较模型组显著升高，有统计学意义（P＜0.001）。各治疗组间相比，血清IL-1β水平在针刺组中最高，在表面激光针灸50 mW组中最低，二者差异有统计学意义（P＜0.05）；血清IL-15水平在针刺组中最高，在药物组中最低，差异无统计学意义（P＞0.05）；血清IL-17水平在深部激光针灸1 mW组中最高，在表面激光针灸100 mW组中最低，差异有统计学意义（P＜0.001）；血清TNF-α水平在深部激光针灸1 mW组中最高，在药物组中最低，差异有统计学意义（P＜0.001）；血清VEGF水平在深部激光针灸50 mW组中最高，在深部激光针灸5 mW组中最低，差异有统计学意义（P＜0.05）；血清COR水平在针刺组中最低，在表面激光针灸150 mW组中最高，差异有统计学意义（P＜0.001）。激光针灸能够有效降低CIA大鼠血清IL-1β、IL-15、IL-17、TNF-α、VEGF等促炎因子的表达水平，提高血清COR等抗炎因子的表达水平，其作用效果与激光针灸的作用方式和参数设置有关。

We study the impact of laser acupuncture with different methods and parameters on type-II collagen-induced arthritis (CIA) model rat serum interleukin-1β (IL-1β), interleukin-15 (IL-15), interleukin-17 (IL-17), tumor necrosis factor α (TNF-α), vascular endothelial growth factor (VEGF) and cortisol (COR). The rats are randomly divided into model group, medication group, acupuncture group, deep laser acupuncture 1 mW group, 5 mW group, 10 mW group, the surface laser acupuncture 50 mW group, 100 mW group, 150 mW group, and normal control group is set, selecting Zusanli and Shenshu as acupuncture points. Samples are selected after 10 days of treatment. We use double-antibody enzyme-linked immunosorbent sandwich assay (ELISA) to measure serum cytokine level. Serum IL-1β, IL-15, IL-17, TNF-α and VEGF in the model group are significantly higher than those in the normal group (all P<0.001), and COR is significantly lower than the normal group (P<0.001). IL-1β, IL-15, IL-17, TNF-α and VEGF in all treatment groups after treatment are reduced to different degrees compared with model group, and except individual groups, there are statistical differences (P<0.05, P<0.01, P<0.001); COR in each treatment group is significantly higher than that in the model group (P<0.001). Compared among treatment groups, serum IL-1β level is the highest in the acupuncture group and the lowest in the surface laser acupuncture 50 mW group, and the two have statistical difference (P<0.05); serum IL-15 level is the highest in the acupuncture group and the lowest in the drug group, but the difference is not statistical (P>0.05); serum IL-17 level is the highest in the deep laser acupuncture 1 mW group and the lowest in the surface laser acupuncture 100 mW group, and the difference is statistical (P<0.001); serum TNF-α level is the highest in deep laser acupuncture 1 mW group and the lowest in drug group, and the difference is statistical (P<0.001); serum VEGF level is the highest in deep laser acupuncture 50 mW group and the lowest in the deep laser acupuncture 5 mW group, and the difference is statistical (P<0.05); serum COR level is the lowest in the acupuncture group, and the highest in surface laser acupuncture 150 mW group, the difference is statistical (P<0.001). Laser acupuncture can effectively reduce CIA rat serum proinflammatory cytokines levels such as IL-1β, IL-15, IL-17, TNF-α and VEGF, increase serum anti-inflammatory cytokines levels such as COR, and its effect is related with action mode of laser acupuncture and parameter settings.