多焦点多光子显微技术(multifocal multiphoton microscopy, MMM)提高了激发光能的利用率和成像速度, 可以实现样品的三维快速多光子激发荧光显微成像, 并具有对活体样品损伤小, 成像深度大, 图像信噪比高等优点。详细阐述了MMM的实现方法及其研究进展, 包括同时时间和光谱分辨的MMM(simultaneous time-and spectrum-resolved multifocal multiphoton microscopy, STSR-MMM)、基于时间相关单光子计数技术的MMM(multifocal multiphoton microscopy based on time-correlated single photon counting, TCSPC-MMM)、基于随机扫描的MMM (stochastic scanning multifocal multiphoton microscopy, SS-MMM)、基于固定光路系统的变视场扫描的MMM等技术。

Multifocal multiphoton microscopy (MMM) can greatly improve the utilization of the excitation light and imaging speed, and it can also acquire three-dimensional fluorescence microscopic images by multiphoton excitation with the advantages of high speed, reduced photobleaching and photodamage, enhanced penetration depth and high signal to noise ratio. The realization and research progresses of multifocal multiphoton microscopy are discussed in detail, which include simultaneous time-and spectrum-resolved multifocal multiphoton microscopy(STSR-MMM), multifocal multiphoton microscopy based on time-correlated single photon counting(TCSPC-MMM), multifocal multiphoton microscopy using a field of view zoom scanning protocol and stochastic scanning multifocal multiphoton microscopy(SS-MMM), etc.