Journal of Innovative Optical Health Sciences, 2017, 10 (3): 1750008, Published Online: Dec. 27, 2018  

Cell counting for in vivo flow cytometry signals with baseline drift

Author Affiliations
1 Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai 200030, P. R. China
2 School of Naval Architecture, Ocean and Civil Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China
Abstract
In biomedical research fields, the in vivo flow cytometry (IVFC) is a widely used technology which is able to monitor target cells dynamically in living animals. Although the setup of IVFC system has been well established, baseline drift is still a challenge in the process of quantifying circulating cells. Previous methods, i.e., the dynamic peak picking method, counted cells by setting a static threshold without considering the baseline drift, leading to an inaccurate cell quantification. Here, we developed a method of cell counting for IVFC data with baseline drift by interpolation fitting, automatic segmentation and wavelet-based denoising. We demonstrated its performance for IVFC signals with three types of representative baseline drift. Compared with non-baseline-correction methods, this method showed a higher sensitivity and specificity, as well as a better result in the Pearson's correlation coe±cient and the mean-squared error (MSE).

Xiaoling Wang, Yuanzhen Suo, Dan Wei, Hao He, Fan Wu, Xunbin We. Cell counting for in vivo flow cytometry signals with baseline drift[J]. Journal of Innovative Optical Health Sciences, 2017, 10(3): 1750008.

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