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Side lobe supression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging

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摘要

The fluorescence from out-of-focus region excited by the side lobes of Bessel beam is the major concerns for the light-sheet fluorescence microscopy (LSFM) with Bessel beam plane illumination. Here, we propose a method of applying the subtractive imaging to overcome the limitation of the conventional LSFM with Bessel beam plane illumination. In the proposed method, the sample is imaged twice by line-scanning using the extended solid Bessel beam and the ring-like Bessel beam. By subtracting between the two images with similar out-of focus blur, the improved image quality with the suppression of the Bessel beam side lobes and enhanced sectioning ability with improved contrast is demonstrated.

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作者单位:

    Nanchang University
    Nanchang University
    Nanchang University
    Nanchang University
    Nanchang University

引用该论文

Deng Suhui,Xiao Yiping,Hu Jie,Chen Jianfang,Wang Yuhao,Liu Mingping. Side lobe supression in light-sheet fluorescence microscopy with Bessel beam plane illumination using subtractive imaging[J].Chinese Optics Letters,2018,16(11):11.