皮肤胆固醇无创光谱检测模拟和在体实验研究

候华毅; 方朝晖; 张元志; 董美丽; 朱灵; 王贻坤; 叶兴顺; 桑瑶烁; 刘勇

doi:doi:10.3788/cjl201643.0907001

中国激光, 2016, 43 (9): 0907001, 网络出版: 2018-05-25

皮肤胆固醇无创光谱检测模拟和在体实验研究下载： 531次

Simulation and in vivo Experimental Study on Noninvasive Spectral Detection of Skin Cholesterol

论文大纲

候华毅 ^1,2方朝晖 ³张元志 ¹董美丽 ^1,*朱灵 ¹王贻坤 ¹叶兴顺 ¹桑瑶烁 ¹刘勇 ¹

作者单位

¹ 中国科学院合肥物质科学研究院应用技术研究所安徽省生物医学光学仪器工程技术研究中心，安徽合肥 230088

² 中国科学技术大学研究生院科学岛分院，安徽合肥 230026

³ 安徽中医药大学第一附属医院内分泌科，安徽合肥 230031

医用光学皮肤胆固醇漫反射光谱无创检测动脉粥样硬化 medical optics skin cholesterol diffuse reflectance spectrum noninvasive detection atherosclerosis

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摘要

皮肤胆固醇是评估动脉粥样硬化性疾病风险的新型生物标志物。为实现皮肤胆固醇的无创、快速检测，设计了一套基于漫反射光谱技术的检测系统。采用毛地黄皂苷-辣根过氧化物酶共聚物溶液模拟梯度浓度皮肤胆固醇。通过检测该共聚物溶液和受试者在体皮肤胆固醇，验证了系统的可行性。结果表明，在440~550 nm特征波段内，由相对漫反射率引入的参数S值与共聚物溶液浓度呈线性关系（相关系数r=0.992，P<0.01）；控制年龄、性别等因素后，受试者在体皮肤胆固醇无创检测结果与血液中总胆固醇和低密度脂蛋白胆固醇呈显著正相关，相关系数r分别为0.837（P<0.01）和0.778（P<0.01）。漫反射光谱技术为皮肤胆固醇的检测提供了一种无创、便捷的方法。在体皮肤胆固醇的无创检测有助于动脉粥样硬化性疾病的早期发现。

Abstract

Skin cholesterol is a novel biomarker to assess the risk of atherosclerotic diseases. A system based on diffuse reflectance spectroscopy technology is designed for noninvasive and rapid detection of skin cholesterol. The feasibility of the system is validated through detecting digitonin-horseradish peroxidase copolymer solution which simulates the skin cholesterol of gradient concentration and skin cholesterol in vivo of subjects. A parameter S based on the relative diffuse reflectance increases linearly with the increasing concentration of the copolymer solution in the wavelength band from 440 nm to 550 nm (correlation coefficient r=0.992, P<0.01). After adjusting age, gender and other factors, it shows significant positive correlation between the skin cholesterol test results of subjects and the total cholesterol, low density lipoprotein cholesterol, and the correlation coefficients are 0.837 (P<0.01) and 0.778 (P<0.01), respectively. The diffuse reflectance spectroscopy is a noninvasive and convenient method for the detection of skin cholesterol, and the noninvasive detection of skin cholesterol in vivo contributes to the early detection of atherosclerotic diseases.

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候华毅, 方朝晖, 张元志, 董美丽, 朱灵, 王贻坤, 叶兴顺, 桑瑶烁, 刘勇. 皮肤胆固醇无创光谱检测模拟和在体实验研究[J]. 中国激光, 2016, 43(9): 0907001. Hou Huayi, Fang Zhaohui, Zhang Yuanzhi, Dong Meili, Zhu Ling, Wang Yikun, Ye Xingshun, Sang Yaoshuo, Liu Yong. Simulation and in vivo Experimental Study on Noninvasive Spectral Detection of Skin Cholesterol[J]. Chinese Journal of Lasers, 2016, 43(9): 0907001.

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