研制了一种可探测细胞微弱荧光图像的数字化高灵敏度荧光显微镜，采用视频数字化增强型CCD系统作为高灵敏度的接收系统，像增强器亮度增益为41，000，因而这种荧光显微镜可探测到普通荧光显微镜不能观察的微弱荧光图像，并可减少荧光物质的浓度和激发光的强度，减少对细胞自然生理环境的影响。数字化高灵敏度荧光显微镜在给出细胞微弱荧光图像的同时，并可给出图像上每一像元的发光强度和细胞平均发光强度。使用此仪器已首次直接得到了：1)光敏竹红菌甲素(HA)在HeLa细胞(人体宫颈癌细胞)中的分布，证实HA主要分布在HeLa的细胞膜中；2)HeLa细胞在有HA存在时受强光照射后的光损伤现象；3)加入三种细胞抗氧化剂维生素E、丁羧基甲苯(SF)和阿魏酸钠(BHT)后获得不同程度保护的细胞状态；4)一定浓度的HA在HeLa细胞随时间的摄取曲线；5)不同HA浓度一定时间内在HeLa细胞中被吸收量的曲线。

A digitalized ultra sensitive fluorescence microscope has been developed, in which an intensifier CCD is used as high sensitive image detector with luminance gain of 41,000. It can detect ultra weak fluorescence image and decrease the concentration of fluorescence label and emitting light intensity in order to keep natural situation of the detected cell. This digitalized microscope can be used not only to obtain the fluorescence image, but also get the light luminance at every point of the image and the average light luminance of the whole image quantitatively. With this instrument, we have obtained: 1) The image of hypocrellin-A distribution in HeLa cell, it shows the HA is mainly absorbed by cell membrance; 2) The image of HeLa cell be oxidated by strong light after adding HA; 3) Images of HeLa cel being protected by three antioxidant reagent Vit. E, sodium ferulate (SF) and butylated hydroxytoluene (BHT), from which it has been shown that anti-oxidated effect of Vit E is the best; 4) The curve of absorbed quantity of HA in a HeLa cell with time versus culture time; 5) The curve of absorbed quantity of HA in Hela cell versus concentration of HA solution.