基于可编程LED阵列照明的透射体视显微镜

孔富城; 张玉珍; 孙佳嵩; 陈钱; 左超

doi:doi:10.3788/aos201636.0511005

光学学报, 2016, 36 (5): 0511005, 网络出版: 2016-05-03

基于可编程LED阵列照明的透射体视显微镜下载： 535次

Transmission Stereo Microscope Based on Programmable LED Array Illumination

论文大纲

孔富城 ^1,2,*张玉珍 ²孙佳嵩 ^1,2陈钱 ²左超 ^1,2

作者单位

¹ 南京理工大学电子工程与光电技术学院智能计算成像实验室，江苏南京 210094

² 南京理工大学电子工程与光电技术学院江苏省光谱成像与智能感知重点实验室，江苏南京 210094

成像系统显微镜三维图像可编程照明发光二极管阵列 imaging systems microscopy three-dimensional image programmable illumination light emitting diode array

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摘要

为了实现对待测样品高质量的体视显微成像，提出了基于可编程发光二极管（LED）阵列照明的透射体视显微镜。该显微镜光学系统使用可编程LED阵列作为照明双光源，照明孔径、角度以及波长均可自由选择，通过改变可编程LED阵列上红蓝圆形光源的半径r和圆心间距d分别实现对体视显微镜焦深和体视角的灵活可控。实验结果表明，该显微镜系统简便，观察者根据自身需要选择合适的焦深和体视角可有效解决因人眼瞳距个体差异大引起单一体视角出现的双像不能重合等问题，实现对待测样品的直接体视显微观看。使用数值孔径NA=0.1(4×)的显微物镜，在最佳参数r=2，d=3时可清晰观测到待测样品的层次关系以及相对位置等三维信息。

Abstract

A transmission stereo microscope is presented based on programmable light emitting diode（LED） array illumination so as to realize stereo imaging with high quality. The programmable LED array is the illumination source of this optical system. The radius, angle and wavelength of illumination can be chosen freely. The depth of focus and the stereotype angle can be adjusted by changing r and d of the programmable LED array at will. Experimental results show that the microscope system is simple, and double images which can not be coincided owning to the difference of individual pupil distance can be solved effectively by choosing appropriate r and d. The stereo image of samples can be obtained in details directly. The three-dimensional information such as hierarchy and relative position can be observed with optimal parameters r=2 and d=3 by using objective NA=0.1(4×).

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孔富城, 张玉珍, 孙佳嵩, 陈钱, 左超. 基于可编程LED阵列照明的透射体视显微镜[J]. 光学学报, 2016, 36(5): 0511005. Kong Fucheng, Zhang Yuzhen, Sun Jiasong, Chen Qian, Zuo Chao. Transmission Stereo Microscope Based on Programmable LED Array Illumination[J]. Acta Optica Sinica, 2016, 36(5): 0511005.

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