质子转移反应飞行时间质谱的建立与性能研究

介绍了自主研制的质子转移反应飞行时间质谱的基本结构和性能。首先对数据采集卡甄别离子脉冲的阈值电压进行了优化,结果表明当阈值电压为0.33V时, H316O+ (m/z 19)与H318O+ (m/z 21) 的离子计数比约500:1;然后考察了水团簇离子H3O+ (H2O)n=0,1,2,3的分布与漂移管约化电场的关系, 结果表明,当约化电场(E/N)为139 Td(1 Td=10－17 Vcm2)时,团簇离子得到很好地抑制,质谱观察到的离子主要是H3O+,其纯度可达99%以上；随后考察了质谱的分辨率,结果表明在m/z=124处,分辨率最好,达到2653; 采用不同浓度的乙醇标样对仪器的线性范围和检测限性能进行了测试,结果表明该仪器的线性动态范围可达三个数量级, 检测限可达3.8 ppb;最后用该质谱仪检测了实验室空气,得到了实验室空气的质谱图。自主研制的质子转移反应飞行时间质谱可实现对痕量挥发性有机物的实时在线测量,在环境、食品、医学等领域具有重要应用价值。

Abstract

The construction and the performance of the newly developed proton transfer reaction time of flight mass spectrometer (PTR-TOFMS) are reported. Firstly, the threshold voltage of the discriminator on data acquisition board was optimized. The results show that when the threshold voltage is 0.33 V, the intensity ratio of H316O+ (m/z 19) and H318O+ (m/z 21) is about 500:1. Then the relationship between the distribution of water clusters ion H3O+ (H2O)n=0,1,2,3 and the reduced electric field in drift tube was studied. The results show that when reduced-field (E/N) is 139 Td (1 Td=10－17 Vcm2), the water cluster ions are well suppressed, and the purity of the ion H3O+ is more than 99%. The resolutions at different m/z were also investigated. The results show that the best resolution is at m/z=124 with RMAX=2653. The performance of the PTR-TOFMS instrument was tested by using different concentrations of ethanol gas. The results show that the linear dynamic range of the PTR-TOFMS instrument can reach three orders of magnitude, and the detection limit can reach 3.8 ppb. At last, the PTR-TOFMS was used to detect the laboratory air to get a mass spectrum of different volatile organic compounds (VOCs). It is indicated that the newly developed PTR-TOFMS can be used for on-line monitoring trace volatile organic compounds, which will be of great value in the fields of environment, food, medicine and other fields.

参考文献

[1] Lindinger W. Hansel A. Analysis of trace gases at ppb levels by proton transfer reaction mass spectrometry (PTR-MS)[J].Plasma Sources Science & Technology, 1997. 6(2):111-117.

[2] Yuan B., Koss A. R., Warneke C.,et al. Proton-Transfer-Reaction mass spectrometry: Applications in atmospheric sciences [J]. Chemical Reviews, 2017. 117(21):13187-13229.

[3] Hewitt C. N.,Hayward S. Tani A. The application of proton transfer reaction-mass spectrometry (PTR-MS) to the monitoring and analysis of volatile organic compounds in the atmosphere [J].Journal Of Environmental Monitoring, 2003. 5(1):1-7.

[4] 金顺平,李建权,韩海燕,等.质子转移反应质谱在线检测痕量挥发性有机物[J]化学进展, 2007. 19(6): 996-1006.

Jin Shunping,Li Jianquan,Han Haiyan,et al. Proton transfer reaction mass spectrometry for online detection of trace volatile organic compounds [J]. Progress In Chemistry, 2007. 19(6):996-1006(in Chinese).

[5] Zou Xue, Kang Meng, Li Aiyue,et al. Spray inlet proton transfer reaction mass spectrometry (SI-PTR-MS) for rapid and sensitive online monitoring of benzene in water [J]. Analytical Chemistry, 2016. 88(6):3144-3148.

[6] Kang Meng, Zou Xue, Lu Yan,et al. Application of a self-developed proton transfer eaction-Mass spectrometer to on-line monitoring trace volatile organic compounds in ambient air [J]. Chemical Research In Chinese Universities, 2016. 32(4): 565-569.

[7] Romano A.,Capozzi V.,Spano G.,et al. Proton transfer reaction-mass spectrometry: online and rapid determination of volatile organic compounds of microbial origin [J]. Applied Microbiology And Biotechnology, 2015. 99(9): 3787-3795.

[8] Zhou Wenzhao, Huang Chaoqun,Zou Xue,et al. Exhaled breath online measurement for cervical cancer patients and healthy subjects by proton transfer reaction mass spectrometry [J]. Analytical And Bioanalytical Chemistry, 2017. 409(23): 5603-5612.

[9] Zou Xue, Zhou Wenzhao,Lu Yan,et al. Exhaled gases online measurements for esophageal cancer patients and healthy people by proton transfer reaction mass spectrometry [J]. Journal Of Gastroenterology And Hepatology, 2016. 31(11): 1837-1843.

[10] Capozzi Vittorio, Yener Sine, Khomenko Iuliia,et al. PTR-ToF-MS coupled with an automated sampling system and tailored data analysis for food studies: Bioprocess Monitoring, Screening and Nose-space Analysis [J]. Jove-Journal Of Visualized Experiments, 2017(123).

[11] Perez-Hurtado P., Palmer E., Owen T.,et al. Direct analysis of volatile organic compounds in foods by headspace extraction atmospheric pressure chemical ionisation mass spectrometry [J]. Rapid Commun Mass Spectrom, 2017. 31(22):1947-1956.

[12] 李建权,沈成银,王鸿梅,等.质子转移反应质谱的建立与性能研究[J].分析化学, 2008(01):132-136.

Li Jianquan., Shen Chenyin., Wang Hongmei.,et al. Development and validation of proton transfer reaction mass spectrometry [J]. Chinese Journal of Analytical Chemistry, 2008(01): 132-136(in Chinese).

[13] Shen Chengyin, Niu Wenqi, Huang Chaoqun,et al. Proton-extraction-reaction mass spectrometry (PER-MS) for monitoring organic and inorganic compounds [J]. International Journal of Mass Spectrometry, 2014. 371: 36-41.

[14] Pan Yue,Zhang Qiangling,Zhou Wenzhao,et al. Detection of Ketones by a novel technology: Dipolar proton transfer reaction mass spectrometry (DP-PTR-MS) [J]. Journal Of the American Society for Mass Spectrometry, 2017. 28(5): 873-879.

[15] de Gouw J. Warneke C. Measurements of volatile organic compounds in the earths atmosphere using proton-transfer-reaction mass spectrometry [J].Mass Spectrometry Reviews, 2007. 26(2): 223-257.

[16] Asaduzzaman Mohammad, Biasioli Franco, Cosio Maria Stella,et al. Hexanal as biomarker for milk oxidative stress induced by copper ions [J]. Journal Of Dairy Science, 2017. 100(3): 1650-1656.

[17] Samudrala D., Brown P. A., Mandon J.,et al. Optimization and sensitive detection of sulfur compounds emitted from plants using proton transfer reaction mass spectrometry [J]. International Journal Of Mass Spectrometry, 2015. 386:6-14.

梁渠, 张亚婷, 张强领, 邹雪, 陈美玲, 王鸿梅, 黄超群, 沈成银, 储焰南. 质子转移反应飞行时间质谱的建立与性能研究[J]. 大气与环境光学学报, 2019, 14(4): 289. LIANG Qu, ZHANG Yating, ZHANG Qiangling, ZOU Xue, CHEN Meiling, WANG Hongmei, HUANG Chaoqun, SHEN Chengyin, CHU Yannan. Development and Validation of Proton Transfer Reaction Time of Flight Mass Spectrometer[J]. Journal of Atmospheric and Environmental Optics, 2019, 14(4): 289.

质子转移反应飞行时间质谱的建立与性能研究

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