中国激光, 2020, 47 (2): 0207016, 网络出版: 2020-02-21
生物医学光声显微成像:技术和应用进展 
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Biomedical Photoacoustic Microscopy: Advances in Technology and Applications
图 & 表
图 2. PAM成像系统的典型实现方式[1,17]。(a)透射式OR-PAM;(b)反射式 OR-PAM;(c)基于暗场照明的 AR-PAM;(d)反射式OR-PAM 典型系统示意图
Fig. 2. Typical embodiments of PAM[1,17]. (a) Transmission-mode OR-PAM; (b) reflection-mode OR-PAM; (c) dark-field AR-PAM; (d) schematic of typical OR-PAM
图 3. 超分辨光声显微图像[25-26]。(a)直径为150 nm的金纳米颗粒的超分辨率PAM图像[25];(b)成纤维细胞中线粒体的传统PAM图像[26];(c)成纤维细胞中线粒体的超分辨率PAM图像[26];(d)成纤维细胞中线粒体的扫描隧道电子显微镜图像[26]
Fig. 3. Super-resolution PAM imaging[25-26]. (a) Photoimprint-PAM imaging of gold nanoparticles with a diameter of 150 nm[25]; (b) imaging of mitochondria in fibroblasts by conventional PAM[26]; (c) imaging of mitochondria in fibroblasts by super-resolution PAM; (d) imaging of mitochondria in fibroblasts by transmissi
图 4. 空间分辨率不变的光声显微成像技术的基本原理[51]
Fig. 4. Principle of spatially invariant resolution photoacoustic microscopy[51]
图 5. PAM/OCT双模态眼底成像[4]。(a)系统示意图;(b)活体新西兰白兔的眼底视网膜照片;(c)视网膜光声图像;(d)三维渲染图像;(e)脉络膜光声图像;(f)眼底的OCT行扫描图像(B-scan)
Fig. 5. Integrated PAM/OCT for multi-modal chorioretinal imaging[4]. (a) System schematic; (b) fundus photo of retina vessels in New Zealand albino rabbit in vivo; (c) PAM image of retinal vessels; (d) three-dimensional rendered image of retinal vessels; (e) PAM image of choroidal vessels; (f) OCT B-scan of the fundus
图 6. 离体黑色素瘤细胞[21]和红细胞[103-104]的光声图像。(a)黑色素瘤细胞的光声图像[21];(b)黑色素瘤细胞的明场图像[21];(c)细胞核经染色后的荧光图像与光声图像的合成[21];(d)红细胞的光声图像[103];(e)红细胞的明场图像[21] and a red blood cell[103-104]in vitro. (a) PAM image of a melanoma cell[21]; (b) bright field microscopy image of a melanoma cell[21]; (c) fluorescence image of the cell nuclei superimposed with the PAM image[
图 7. PAM功能成像。(a)小鼠大脑中单个红细胞的氧气释放过程[114];(b)小鼠耳部无标记多参数光声显微图像[113],分别包括血红蛋白总浓度、血氧饱和度、血流速度,以及虚线所示截面上的血流速度;(c)活体小鼠大脑中的血氧饱和度分布[52]
Fig. 7. Functional PAM imaging. (a) Sequential snapshots of single RBCs releasing oxygen in a mouse brain[114];(b) label-free multi-parameter PAM imaging in vivo[113]: total concentration of hemoglobin (CHb), sO2, blood flow, and profile of blood flow speed across the dashed line, respectively; (c) sO2 distribution in a mouse brain[
图 8. 基于可激活光子纳米探针簇的高选择、高灵敏光声细胞成像[3]。(a)基于分裂绿色荧光蛋白(GFP)的团簇纳米探针制备过程; (b)基于团簇分子探针的 U2OS 细胞的高灵敏度光声细胞成像
Fig. 8. Cellular imaging using activatable photonic nanoclusters. (a) Fabrication of nanoclusters based on split green fluorescent protein (GFP) fragments; (b) highly-sensitive PAM images of U2OS cells based on nanoclusters
表 1OR-PAM及AR-PAM的性能指标[6,20]
Table1. Performance of OR-PAM and AR-PAM[6,20]
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表 2不同扫描方法的对比
Table2. Comparison of different scanning methods
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龙晓云, 田超. 生物医学光声显微成像:技术和应用进展[J]. 中国激光, 2020, 47(2): 0207016. Long Xiaoyun, Tian Chao. Biomedical Photoacoustic Microscopy: Advances in Technology and Applications[J]. Chinese Journal of Lasers, 2020, 47(2): 0207016.
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