Robust and rapidly tunable light source for SRS/CARS microscopy with low-intensity noise

Heiko Linnenbank; Tobias Steinle; Florian Mörz; Moritz Flöss; Han Cui; Andrew Glidle; Harald Giessen

doi:doi:10.1117/1.AP.1.5.055001

Advanced Photonics, 2019, 1 (5): 055001, Published Online: Sep. 25, 2019

Robust and rapidly tunable light source for SRS/CARS microscopy with low-intensity noise Download： 759次

Heiko Linnenbank ^1,2,*Tobias Steinle ^1,2Florian Mörz ^1,2Moritz Flöss ²Han Cui ³Andrew Glidle ³Harald Giessen ^1,2

Author Affiliations

¹ SI Stuttgart Instruments GmbH, Öhringen, Germany

² University of Stuttgart, 4th Physics Institute and Research Center SCoPE, Stuttgart, Germany

³ University of Glasgow, College of Science and Engineering, Division of Biomedical Engineering, Glasgow, United Kingdom

Figures & Tables

Fig. 1. (a) Schematic experimental setup. An Yb-solid-state oscillator allows synchronous generation of the Raman Stokes and pump beams. Using an etalon, the narrowband Stokes beam with picosecond pulse duration is obtained, whereas the Raman pump beam is generated by pumping an OPO, which is subsequently frequency doubled in a periodically poled crystal employing the effect of spectral compression. Using this system, CARS and SRS spectroscopies are both feasible, as depicted in (b) and (c).

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Fig. 2. (a), (b) Spectral range and typical output power of the broadband Raman laser system. (c) By employing an etalon, the Stokes beam reaches a bandwidth of 0.71 nm at 1043.1 nm. The original solid-state oscillator spectrum is indicated in gray. (d) Typical spectrum within the Raman pump spectral range. Due to spectral compression during the SHG, the spectrum typically reaches a bandwidth between 0.5 and 1.2 nm.

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Fig. 3. (a) Autocorrelation traces of the Raman pump beam between 750 and 940 nm. Nearly Fourier-limited pulses are observed over the entire Raman pump tuning range, exhibiting typical pulse durations of about 1.1 ps. (b) Autocorrelation of the Stokes beam. (c) Long-term power stability of the Stokes, OPO, and Raman pump (SHG) beams. Fluctuations below 0.5% rms are observed within the Raman pump spectral range, achieving excellent long-term stability. (d) Corresponding relative intensity noise measurements. The Stokes beam reaches the shot-noise limit at a photocurrent of 1.5 mA at about 150 kHz, whereas the OPO and the SHG (Raman pump) exhibit slightly higher noise. All beams are shot-noise limited above 1 MHz, enabling low-noise coherent Raman spectroscopy.

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Fig. 4. (a) SRS spectrum of a 1:1 volume mixture of $D_{2} O$ and $H_{2} O$ recorded by employing the whole tuning range of the presented system. (b) SRG spectrum of acetone showing the characteristic resonance at $2923 {cm}^{- 1}$ . A spontaneous Raman spectrum is overlaid for comparison. The individual measurement points are connected as guide to the eye.

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Fig. 5. (a) $94 μ m \times 94 μ m$ raster scan image of $6 - μ m$ diameter polymer beads. The contrast mechanism is the scattering by the beads, which is lowering the DC-value of the detected Stokes beam. (b) SRG signal images from the raster scan in (a), recorded for a Stokes-pump energy difference of $3067 {cm}^{- 1}$ (PS-band) and $2953 {cm}^{- 1}$ (PMMA-band), revealing two different kinds of beads. (c) Signal-to-noise ratio extracted from images taken at $2953 {cm}^{- 1}$ as a function of the pixel integration time. (d) SRG signal images recorded at $2953 {cm}^{- 1}$ with ascending pixel integration times.

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Heiko Linnenbank, Tobias Steinle, Florian Mörz, Moritz Flöss, Han Cui, Andrew Glidle, Harald Giessen. Robust and rapidly tunable light source for SRS/CARS microscopy with low-intensity noise[J]. Advanced Photonics, 2019, 1(5): 055001.

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