High-speed 3D imaging based on structured illumination and electrically tunable lens

Dongping Wang; Yunlong Meng; Dihan Chen; Yeung Yam; Shih-Chi Chen

doi:doi:10.3788/COL201715.090004

Chinese Optics Letters, 2017, 15 (9): 090004, Published Online: Jul. 19, 2018

High-speed 3D imaging based on structured illumination and electrically tunable lens Download： 1049次

Dongping Wang ^1,2Yunlong Meng ^1,2Dihan Chen ^1,2Yeung Yam ^1,**Shih-Chi Chen ^1,2,*

Author Affiliations

¹ Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China

² Shenzhen Research Institute, The Chinese University of Hong Kong, Shenzhen 518057, China

Figures & Tables

Fig. 1. Optical configuration of the DMD-based SIM system. L1, L2, and L3, collimating lenses; M, high-reflectivity mirror; DM, dichroic mirror.

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Fig. 2. (a) Optical models and ray-tracing analyses of the ETL and objective lens in ZEMAX, (b) NA variation during axial scanning, and (c) PSF variation during axial scanning.

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Fig. 3. (a) Relationship between the focus shift distance and ETL drive current, and (b) relationship between the field magnification and the ETL drive current.

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Fig. 4. Generation of phase-shifted sinusoidal fringes using a DMD: (a) binary patterns programmed to the DMD at three different phases; (b) corresponding illumination patterns captured at the objective focal plane; (c) measured intensity profiles of the illumination patterns along the cut-lines in (b) for the three phases, i.e., $Φ = 0$ , $2 π / 3$ , and $4 π / 3$ .

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Fig. 5. (a) Pollen imaging results from our SIM system versus (b) a regular wide-field microscope. Scalar $bar = 10 μm$ .

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Fig. 6. (a)–(d) Images obtained by the ETL; (e)–(h) images obtained by the precision $z$ -stage. Images of the same depths are grouped in the same row. Scalar $bar = 10 μm$ .

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Dongping Wang, Yunlong Meng, Dihan Chen, Yeung Yam, Shih-Chi Chen. High-speed 3D imaging based on structured illumination and electrically tunable lens[J]. Chinese Optics Letters, 2017, 15(9): 090004.

High-speed 3D imaging based on structured illumination and electrically tunable lens Download： 1049次

Fig. 1. Optical configuration of the DMD-based SIM system. L1, L2, and L3, collimating lenses; M, high-reflectivity mirror; DM, dichroic mirror.

Fig. 2. (a) Optical models and ray-tracing analyses of the ETL and objective lens in ZEMAX, (b) NA variation during axial scanning, and (c) PSF variation during axial scanning.

Fig. 3. (a) Relationship between the focus shift distance and ETL drive current, and (b) relationship between the field magnification and the ETL drive current.

Fig. 5. (a) Pollen imaging results from our SIM system versus (b) a regular wide-field microscope. Scalar $bar = 10 μm$ .

Fig. 6. (a)–(d) Images obtained by the ETL; (e)–(h) images obtained by the precision $z$ -stage. Images of the same depths are grouped in the same row. Scalar $bar = 10 μm$ .

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High-speed 3D imaging based on structured illumination and electrically tunable lens Download： 1049次

Fig. 1. Optical configuration of the DMD-based SIM system. L1, L2, and L3, collimating lenses; M, high-reflectivity mirror; DM, dichroic mirror.

Fig. 2. (a) Optical models and ray-tracing analyses of the ETL and objective lens in ZEMAX, (b) NA variation during axial scanning, and (c) PSF variation during axial scanning.

Fig. 3. (a) Relationship between the focus shift distance and ETL drive current, and (b) relationship between the field magnification and the ETL drive current.

Fig. 5. (a) Pollen imaging results from our SIM system versus (b) a regular wide-field microscope. Scalar bar=10 μm.

Fig. 6. (a)–(d) Images obtained by the ETL; (e)–(h) images obtained by the precision z-stage. Images of the same depths are grouped in the same row. Scalar bar=10 μm.

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Fig. 5. (a) Pollen imaging results from our SIM system versus (b) a regular wide-field microscope. Scalar $bar = 10 μm$ .

Fig. 6. (a)–(d) Images obtained by the ETL; (e)–(h) images obtained by the precision $z$ -stage. Images of the same depths are grouped in the same row. Scalar $bar = 10 μm$ .