光电子快报(英文版), 2016, 12 (2): 93, Published Online: Oct. 12, 2017  

Fabrication and characterization of SPR chips with the modified bovine serum albumin

Author Affiliations
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100090, China
Abstract
A facile surface plasmon resonance (SPR) chip is developed for small molecule determination and analysis. The SPR chip was prepared based on a self assembling principle, in which the modified bovine serum albumin (BSA) was directly self-assembled onto the bare gold surface. The surface morphology of the chip with the modified BSA was investigated by atomic force microscopy (AFM) and its optical properties were characterized. The surface binding capacity of the bare facile SPR chip with a uniform morphology is 8 times of that of the bare control SPR chip. Based on the experiments of immune reaction between cortisol antibody and cortisol derivative, the sensitivity of the facile SPR chip with the modified BSA is much higher than that of the control SPR chip with the un-modified BSA. The facile SPR chip has been successfully used to detect small molecules. The lowest detection limit is 5 ng/mL with a linear range of 5—100 ng/mL for cortisol analysis. The novel facile SPR chip can also be applied to detect other small molecules.
References

[1] Kanda V, Kariuki J K, Harrison D J and McDermott M T, Analytical Chemistry 76, 7257 (2004).

[2] Ming L, Cushing S K and Wu N, Analyst 140, 386 (2014).

[3] Chi L W and Olivo M, Plasmonics 9, 809 (2014).

[4] LI Zhao, YU Xian-tong, QIN Cui-fang, CAO Xiao-dan, PAN Hai-feng and XU Jian-hua, J. Optoelectron. Laser 26, 1423 (2015). (in Chinese)

[5] John M, Sensors 10, 7323 (2010).

[6] de Mol N J, Methods in Molecular Biology 627, 101 (2010).

[7] Liu C, Cui D and Hui L, Biosensors & Bioelectronics 26, 255 (2010).

[8] Kim S J, Gobi K V, Tanaka H, Shoyamac Y and Miura N, Sensors & Actuators B Chemical 130, 281 (2008).

[9] Wijaya E, Lenaerts C, Maricot S, Hastanin J, Habraken S, Vilcot J, Boukherroub R and Szunerits S, Current Opinion in Solid State Materials Science 15, 208 (2011).

[10] QI Jian-xia, J. Optoelectron. Laser 25, 282 (2014). (in Chinese)

[11] DONG Jun, LIU Ji-hong, YAN Xue-wen, ZHANG Wen-wen and ZHANG Bin, J. Optoelectron. Laser 26, 1222 (2015). (in Chinese)

[12] Cui D F, Cai H Y and Wang J B, High Throughput Multi Parameter Image Surface Plasmon Resonance Test Instrument, China patent, 200610066542.4 (2007).

[13] Yu Q, Chen S, Taylor A D, Homola J, Hock B and Jiang S, Sensors & Actuators B Chemical 107, 193 (2005).

[14] M. al’Absi and D. K. Arnett, Biomed. Pharmacother 54, 234 (2000).

[15] Knutsson U, Dahlgren J, Marcus C, Rosberg S, Bronnegard M and Stierna P, J. Clin. Endocrinol. Metab. 82, 536 (1997).

CHEN Xing, ZHANG Lu-lu, CUI Da-fu. Fabrication and characterization of SPR chips with the modified bovine serum albumin[J]. 光电子快报(英文版), 2016, 12(2): 93.

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