64通道单粒子荧光光谱数据采集系统

张寅超; 姚娇娇; 杜宪炟; 陈和; 郭磐

光学技术, 2019, 45 (5): 562, 网络出版: 2020-01-07

64通道单粒子荧光光谱数据采集系统

64-Channels single-particle fluorescence spectrum data acquisition system

论文大纲

张寅超 ^*姚娇娇杜宪炟陈和郭磐

作者单位

北京理工大学光电学院光电成像技术与系统教育部重点实验室, 北京 100081

激光诱导荧光单粒子荧光 64通道PMT阵列 Laser Induced Fluorescence Single-particle fluorescence 64 channels PMT array

摘要

为了提高微弱荧光信号的探测能力, 获取高分辨光谱信息, 通过2套32通道光电倍增管（PMT）阵列, 结合激光诱导荧光技术, 搭建了64通道荧光光谱数据采集系统, 并使用LabVIEW对数据采集系统进行软件控制, 实现光谱数据的高速采集与处理。实验结果表明该采集系统光谱分辨率为4.69nm, 能够对微流控芯片内通过的单粒子样品溶液进行荧光光谱采集, 实现计数功能。

Abstract

In order to improve the detection ability of weak fluorescence signals, acquire high-spectral resolution information, a 64 channels of fluorescence spectral data acquisition system is built by combining two sets of 32-channel photomultiplier tube (PMT) arrays with laser-induced fluorescence technology, and LabVIEW is used for software control of data acquisition system. The acquisition system achieves the high-speed acquisition and processing of spectral data. The experimental results show that the system can perform the fluorescence spectra acquisition of the single-particle sample solution passing through the microfluidic chip and realize the counting function, and the spectral resolution of the acquisition system is 4.69nm.

参考文献

[1] Hill S C, Pinnick R G, Niles S, et al. Real-time measurement of fluorescence spectra from single airborne biological particles［J］. Field Analyt. Chem. Technol,1999,3(4-5):221-239.

[2] 刘方武. 激光诱导荧光微生物检测技术研究［D］. 中国科学院研究生院(上海技术物理研究所),2014:73-77.

Liu Fangwu. Study of the laser induced fluorescence technology for detection microorganisms［D］. University of Chinese Academy of Sciences (Shanghai Institute of Technical Physics),2014:73-77.

[3] Pan Y L, Cobler P, Rhodes S, et al. High-speed, high-sensitivity aerosol fluorescence spectrum detection using a 32-anode photomultiplier tube detector［J］. Review of Scientific Instruments,2001,72(3):1831-1836.

[4] Huang H C, Pan Y L, Hill S C, et al. Real-time measurement of dual-wavelength laser-induced fluorescence spectra of individual aerosol particles ［J］. Optics Express,2008,16(21):16523-16528.

[5] Pan Y L, Hill S C, Pinnick R, et al. Fluorescence spectra of atmospheric aerosol particles measured using one or two excitation wavelengths: comparison of classification schemes employing different emission and scattering results［J］. Optics Express,2010,18(12):12436-12457.

[6] Benda A, Kapusta P, Hof M, et al. Fluorescence spectral correlation spectroscopy (FSCS) for probes with highly overlapping emission spectra［J］. Optics Express,2014,22(3):2973-2988.

[7] 马佑军, 赵朝方, 李晓龙, 等. 多通道激光雷达油荧光光谱探测系统研制与实验分析［C］∥中国光学学会:中国光学学会,2010:9.

Ma Youjun, Zhao Chaofang, Li Xiaolong, et al. Development and experimental andysis of Multi-channel lidar fluorescence spectrol detection system［C］∥Chinese Optical Sciety,Tianjin University,P.C.China,2010:9.

[8] 李静. 基于罗丹明6G荧光探针的合成及其检测应用的研究［D］. 山东大学,2017:1-3.

Li Jing. Studies on the syntheses and dectection applications of rhodamine 6G-based fluorescence probes［D］. Shandong University, 2017:1-3.

[9] 王世立, 方肇伦. 微流控光谱分析进展［J］. 光谱学与光谱分析,2000,20(2):143-148.

Wang Shili, Fang Zhaolun. Developments in microfluidic spectrometry［J］. Spectroscopy and Spectral Analysis,2000,20(2):143-148.

[10] 刘春梅, 王洋, 吴元庆, 等. 应用于生物荧光检测的微流控芯片的研究［J］. 人工晶体学报,2017(06):209-214.

Liu Chunmei, Wang Yang, Wu Qingyuan, et al. Research on micro fluidic chips used for bioliminescence detection ［J］. Journal of Synthetic Crystals, 2017(06):209-214.

张寅超, 姚娇娇, 杜宪炟, 陈和, 郭磐. 64通道单粒子荧光光谱数据采集系统[J]. 光学技术, 2019, 45(5): 562. ZHANG Yinchao, YAO Jiaojiao, DU Xianda, CHEN He, GUO Pan. 64-Channels single-particle fluorescence spectrum data acquisition system[J]. Optical Technique, 2019, 45(5): 562.

64通道单粒子荧光光谱数据采集系统

关于本站 Cookie 的使用提示

全站搜索

64通道单粒子荧光光谱数据采集系统

相关论文

相关资讯

关于本站 Cookie 的使用提示

全站搜索