皖西白鹅胚胎中、后期肌组织发育变化的观察

分别于17、23、29胚龄各取6枚胚胎发育正常的种蛋, 分离鹅胚的心脏、肌胃及大腿骨骼肌, 制作石蜡切片, HE染色, 显微观察、摄影。观察皖西白鹅胚胎中、后期肌肉组织发育的变化。结果表明, 17d时的骨骼肌、平滑肌、心肌的发育程度均较低, 没有形成正常的肌纤维形态, 之后呈现随龄性增长。骨骼肌和平滑肌在17 d～23 d期间是以肌纤维数量的增加为主, 即成肌细胞的分裂增殖, 23 d～29 d 期间成肌细胞分化融合形成肌纤维; 心脏成纤维细胞增殖速度较快, 17 d心脏肌纤维已经排列紧密基本吻合成网状; 17 d后主要是心肌纤维的伸长和成熟化的过程。说明胚胎期心肌组织的生长发育要早于骨骼肌与平滑肌。

Abstract

6 eggs picked out from normal development when the embryonic age were 17, 23, 29 days respectively. The heart, muscular stomach and leg muscle were separated carefully, then made into paraffin sections, stained with HE, microscope observation and photography, to observe the developmental changes of muscle tissue at embryonic middle and later stage of Wanxi White Geese. The results showed that the development degree of skeletal muscle, smooth muscle and cardiac muscle were all lower and had no normal muscle fiber morphology at 17 d, but following muscle tissue development were appeared with age. It was predominated that Skeletal muscle and smooth muscle were to increase the number of muscle fibres from 17 d to 23 d, namely the myoblast’s division and proliferation, and the myoblasts to form muscle fiber pass through differentiation and fusion from 23 d to 29 d.Cardiac fibroblast proliferation speed were faster, cardiac muscle fiber was arranged closely and to form mesh basically at 17 d, myocardial fiber elongation and maturation happened mainly after 17 d.It indicates that the myocardial tissue’ growth and development are earlier than that the skeletal muscle and smooth muscle at embryonic period.

参考文献

[1] 左瑞华, 胡孝东. 对六安市皖西白鹅发展的思考［J］. 黑龙江畜牧兽医, 2004, (6): 87-88.

ZUO Ruihua, HU Xiaodong. Thinking on the development of Wanxi White Geese in Lu'an city［J］. Heilongjiang Animal Science and Veterinary Medicine, 2004, (6): 87-88.

[2] 刘丑生, 赵兴波, 李宁, 等. 动物肌肉生长发育调控的功能基因研究进展［J］. 中国畜牧杂志, 2003, 39(5): 48-49.

LIU Chousheng, ZHAO Xingbo, LI Ning, et al. Progress in the study of gene regulation of the growth and development of animal muscle［J］. Chinese Journal of Animal Science, 2003, 39(5): 48-49.

[3] FAURE S, GEORGES M, MCKEY J, et al. Expression pattern of the homeotic gene Bapx1 during early chick gastrointestinal tract development［J］. Gene Expr Patterns, 2013, 13(8): 287-292.

[4] 张涌, 侯玲玲. 山羊胚胎心肌细胞分化发育的超微结构研究［J］. 西北农林科技大学学报(自然科学版), 2001, 29(2): 21-24.

ZHANG Yong, HOU Lingling. Ultrastructural study on the differentiation and development of goat embryonic myocardial cells［J］. Journal of Northwest A&F University (Natural Science Edition), 2001, 29(2): 21-24.

[5] 王英典, 曾少举, 刘宁, 等译.发育生物学(英国)［M］.北京: 科学出版社, 2006, 9: 9.

WANG Yingdian, ZENG Shaoju, LIU Ning, et al. translated. Developmental Biology( Britain)［M］. Beijing: Science Press, 2006, 9: 9.

[6] 赵茹茜, 崔胜, 江青艳, 等. 动物生理学(第五版)［M］. 北京: 中国农业出版社, 2011, 7: 71.

ZHAO Ruqian, CUI Sheng, JIANG Qingyan, et al. Animal Physiology (The fifth edition)［M］. Beijing: Chinese Agricultural Press, 2011: 7: 71.

[7] 曹爱玲, 曾卫东, 张才乔.鸡胚骨骼肌细胞增殖和融合培养模型的建立［J］.中国畜牧杂志, 2006, 42(9): 13-29.

CAO Ailing, ZENG Weidong, ZHANG Caiqiao. Establishment of culture model on the skeletal muscle cell proliferation and fusion of chicken embryo［J］. Chinese Journal of Animal Science, 2006, 42(9): 13-29.

[8] DAVIS R L, WEINTRAUB H, LASSAR A B. Expression of a single transfected cDNA converts fibroblasts to myoblasts［J］. Cell, 1987, 51(6): 987-1000.

[9] MARICS I, PADILA F, GUILLEMOT J F, et al. FGFR4 signalling is anecessary step in limb muscle differentiation［J］. Development, 2002, 129(19): 4559-4569.

[10] HIRSINGER E, MALAPERT P, DUBRULLE J, et al. Notch signaling acts in post mitotic avian myogenic cell to control MyoD activation［J］. Development, 2001, 128: 107-116.

[11] NOTARNICOLA C, ROULEAU C, LE GUEN L, et al. The RNA-binding protein RBPMS2 regulates development of gastrointestinal smooth muscle［J］. Gastroenterology, 2012, 143(3): 687-697.

[12] 唐娟, 刘艳, 杨光, 等. 小鼠心脏发育的形态学研究［J］. 四川动物, 2009, 28(3): 403-406.

TANG Juan, LIU Yan, YANG Guang, et al. Morphological studies on the heart development of mice［J］. Sichuan Journal of Zoology, 2009, 28(3): 403-406.

吕锦芳, 张蕾, 宁康健, 应如海, 姜锦鹏, 冯保明. 皖西白鹅胚胎中、后期肌组织发育变化的观察[J]. 激光生物学报, 2013, 22(6): 551. LV Jinfang, ZHANG Lei, NING Kangjian, YING Ruhai, JIANG Jinpeng, FENG Baoming. Observation of the Developmental Changes of Muscle Tissue at Embryonic Middle and Later Stage in Wanxi White Geese[J]. Acta Laser Biology Sinica, 2013, 22(6): 551.

皖西白鹅胚胎中、后期肌组织发育变化的观察

关于本站 Cookie 的使用提示

全站搜索

皖西白鹅胚胎中、后期肌组织发育变化的观察

相关论文

相关资讯

关于本站 Cookie 的使用提示

全站搜索