高灵敏磁分离荧光传感法检测黄曲酶毒素B1

曾云龙; 张敏; 易守军; 何盼; 赵敏; 夏晓东; 唐春然

doi:doi:10.3788/gzxb20184711.1128001

光子学报, 2018, 47 (11): 1128001, 网络出版: 2018-12-17

高灵敏磁分离荧光传感法检测黄曲酶毒素B1

Magnetic Separation Fluorescent Sensor for Highly Sensitive Detection of Aflatoxin B1

论文大纲

曾云龙 ^1,*张敏 ¹易守军 ^1,2何盼 ¹赵敏 ¹夏晓东 ¹唐春然 ¹

作者单位

¹ 湖南科技大学化学化工学院, 理论有机和功能分子教育部重点实验室, 精细聚合物可控制备与功能应用湖南省重点实验室, 湖南湘潭 411201

² 湖南科技大学材料科学与工程学院, 湖南湘潭 411201

摘要

以自组装方式制备了Au@Fe3O4/核酸适体/氨基-碳量子点磁性生物纳米复合物, 并提出一种磁分离荧光传感法用于黄曲霉毒素B1的检测.当样品中有黄曲霉毒素B1时, 磁性生物纳米复合物中核酸适体选择性地与黄曲霉毒素B1结合并释放出氨基-碳量子点, 经磁性分离后, 氨基-碳量子点留在溶液中, 体系溶液荧光强度随黄曲霉毒素B1浓度的增大而增强.黄曲霉毒素B1浓度在0.001~1.0 ng/mL范围与溶液荧光强度成良好线性关系, 线性相关系数为0.996 4, 检测限为0.3 pg/mL.该方法利用磁性分离技术, 有效地消除了背景荧光影响, 改善了荧光传感性能.

Abstract

A fluorescent aptasensor was developed and applied to detect aflatoxin B1 based on Au@Fe3O4/aptamers/aminofunctioned carbon quantum dots magnetic nanocomposites which were constructed self-assembly. The selective interactions between aflatoxin B1 and the aptamer in the samples cause release of the aminofunctioned carbon quantum dots which remains in the solution after magnetic separation. A linear fluorescence signal response to aflatoxin B1 concentration is obtained over a wide aflatoxin B1 concentration range of 0.001~1.00 ng/mL with a detection limit of 0.3 pg/mL, and the correlation coefficient is 0.996 4. The performances of the fluorescent sensor are significantly improved as the background fluorescence is effectively removed by magnetic separation.

参考文献

[1] SU A M, NHONG Q M, WANG Y L, et al. Preparation of carbon quantum dots from cigarette filters and its application for fluorescence detection of Sudan I［J］. Analytica Chimica Acta, 2018, 1023: 115-120.

[2] GORYACHEVA I YU, SAPELKIN A V, SUKHORUKOV G B. Carbon nanodots: Mechanisms of photoluminescence and principles of application［J］. Trends in Analytical Chemistry, 2017, 90: 27-37.

[3] ZHAO Z H, ZHANG J Q, WANG Y T, et al. Hydrothermal synthesis of fluorescent nitrogen- doped carbon quantum dots from ascorbic acid and valine for selective determination of picric acid in water samples［J］. Journal of Environmental Analytical Chemistry, 2016, 96, 1402-1413.

[4] LI Y, SHI F P, CAI N, et al. A biosensing platform for sensitive detection of concanavalin A based on fluorescence resonance energy transfer from CdTe quantum dots to graphene oxide［J］. New Journal of Chemistry, 2015, 39: 6092-6098.

[5] YUAN Y S, ZHAO X, QIAO M, et al. Determination of sunset yellow in soft drinks based on fluorescence quenching of carbon dots［J］. Spectro- imica Acta Part A: Molecular and Biomole- cular Spectroscopy, 2016,167: 106-110.

[6] SUN X C, LEI Y. Fluorescent carbon dots and their sensing applications［J］. Trends in Analytical Chemistry, 2017, 89: 163-180.

[7] HOLA K, MARKOVA Z, ZOPPELLARO G, et al. Tailored functionalization of iron oxide nanoparticles for MRI, drug delivery, magnetic separation and immobilization of biosubstances ［J］. Biotechnology Advances, 2015, 33: 1162-1176.

[8] Rocha-Santos T A P. Sensors and biosensors based on magnetic nanoparticles［J］. Trends in Analytical Chemistry, 2014, 62: 28-36.

[9] HE J C, HUANG M Y, WANG D M, et al. Magnetic separation techniques in sample preparation for biological analysis: A review［J］. Journal of Pharmaceutical and Biomedical Analysis, 2014, 101: 84-101.

[10] LIU L, JIN H, SUN L, et al. Determination of aflatoxins in medicinal herbs by high performance liquid chromatography-tandem mass spectrometry［J］. Phytochemical Analysis, 2012,23(5): 469-476.

[11] WEN J, KONG W J, HU Y C, et al. Multi-mycotoxins analysis in ginger and related products by UHPLC-FLR detection and LC-MS /MS confirmation ［J］. Food Control, 2014,43(8): 82-87.

[12] PAEK S H, LEE S H, CHO J H, et al. Development of rapid one-step immuno-chro-matographic assay［J］. Methods, 2000,22(1): 53-60.

[13] VAHID N, AMMAR C, ALI B, et al. Ultrasensitive aflatoxin B1 assay based on FRET from aptamer labelled fluorescent polymer dots to silver nanoparticles labeled with complementary DNA［J］. Microchimica Acta, 2017, 184: 4655-4662.

[14] WANG B, CHEN Y, WU Y, et al. Aptamer induced assembly of fluorescent nitrogen-doped carbon dots on gold nanoparticles for sensitive detection of AFB1［J］. Biosensors & Bioelectronics, 2016, 78, 23- 30.

[15] 姜利英, 周鹏磊, 肖小楠, 等. 基于氧化石墨烯荧光适体传感器的多巴胺检测［J］.发光学报,2016,37(7): 881-886.

JIANG Li-ying, ZHOU Peng-lei, XIAO Xiao-nan, et al. Fluorescent aptamer biosensor for the detection of dopamine with graphene oxide［J］. Chinese Journal of Luminescence, 2016, 37(7): 881-886.

[16] WANG B, CHEN Y F, WU Y Y, et al．Aptamer induced assembly of uorescent nitrogen-doped carbon dots on gold nanoparticles for sensitive detection of AFB1［J］. Biosensors & Bioelectronics, 2016, 78: 23-30.

[17] WANG B, ZHU Q K, LIAO D L,et al. Perylene probe induced gold nanoparticle aggregation［J］. Journal of Materials Chemistry, 2011, 21: 4821- 826.

[18] JIN R C, WU G S, LI Z, et al. What controls the melting properties of DNA-linked gold nanoparticle assemblies［J］. Journal of the American Chemical Society, 2003, 125: 1643-1654.

[19] WANG C, HU T T, WEN Z Q, et al. Concentration-dependent color tunability of nitrogen-doped carbon dots and their application for iron(III) detection and multicolor bioimaging［J］. Journal of Colloid and Interface Science, 2018, 521: 33-41.

[20] GRABOLLE M, SPIELES M, LESNYAK V, et al. Determination of the fluorescence quantum yield of quantum dots: suitable procedures and achievable uncertainties［J］. Analytical Chemistry, 2009, 81: 6285-6294.

[21] SAHA K, AGASTI S S, KIM C Y, et al. Gold nanoparticles in chemical and biological sensing［J］. Chemical Reviews, 2012,112: 2739-2779.

曾云龙, 张敏, 易守军, 何盼, 赵敏, 夏晓东, 唐春然. 高灵敏磁分离荧光传感法检测黄曲酶毒素B1[J]. 光子学报, 2018, 47(11): 1128001. ZENG Yun-long, ZHANG Min, YI Shou-jun, HE Pan, ZHAO Min, XIA Xiao-dong, TANG Chun-ran. Magnetic Separation Fluorescent Sensor for Highly Sensitive Detection of Aflatoxin B1[J]. ACTA PHOTONICA SINICA, 2018, 47(11): 1128001.

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