生物细胞定量相位测量与恢复方法研究进展

张璐; 赵春晖; 康森柏; 赵宏; 张春伟; 袁莉

doi:doi:10.3788/CJL201845.0207009

中国激光, 2018, 45 (2): 0207009, 网络出版: 2018-02-28

生物细胞定量相位测量与恢复方法研究进展下载： 1510次特邀综述

Progress on Methods of Quantitative Phase Measurement and Retrieval for Biological Cells

张璐 ^1,*赵春晖 ¹康森柏 ¹赵宏 ¹张春伟 ¹袁莉 ²

作者单位

¹ 西安交通大学机械学院，陕西西安 710049

² 西安交通大学第一附属医院检验科，陕西西安 710049

图 & 表

图 1. 同步相移剪切干涉光路示意图[3-4]

Fig. 1. Experimental setup of simultaneous phase shift shear interference[3-4]

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图 2. 离轴数字全息光路示意图。(a)文献[ 10];(b)文献[ 12];(c)文献[ 13];(d)文献[ 15];(e)文献[ 16]

Fig. 2. Schematic of off-axis digital holographic. (a) Ref. [10]; (b) Ref. [12]; (c) Ref. [13]; (d) Ref. [15]; (e) Ref. [16]

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图 3. 基于流体聚焦技术的光路示意图[44]

Fig. 3. Experimental setup of optofluidic time-stretch quantitative phase microscope[44]

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图 4. (a)免显微透镜的共光路相位显微装置[48];(b)多波长免透镜成像显微装置[49];(c)数字全息与全息光镊相结合的光路示意图[50]

Fig. 4. (a) Experimental setup of phase microscopy imaging based on common-path without micro-objective[48];(b) multi-wavelength lens-free video microscopy[49]; (c) schematic of the combined DHM and HOT workstation using holographic optical stretching and quantitative phase imaging of RBCs[50]

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表 1动态生物细胞相位测量技术

Table1. Phase measurement methods for dynamic biological cells

Technology	Year	Sample	Phaseshift	Digitalholographic	Characteristic	Status of sampleandapplication
Polarizing coupledinterferometers	2015	Acetate sheet;red cells	√			Dynamic
Spatial light interferencemicroscopy	2017	Neuronal cells	√			3D dynamic
Simultaneous phase-shiftinterference microscopy	2015		√		Resolution:0.4868 μm	Dynamic
Quantitative phasecytometryline-focusedbeam illumination	2017	Hela cells		√	Imaging speed:1000/13 min^-1;classificationaccuracy: 96.5%	Dynamic
Holographicimaging cytometry	2017	Humanosteosarcoma cells		√	Duration: 2 d	Dynamic
Low-coherenceoff-axis interferencephase microscopy	2017	Melanoma cells;normal cells		√	Classificationaccuracy: 81%-99%	3D dynamic
Digital holographicmicroscopy	2017	Hela cells		√	Duration: 2-3 d	4D dynamic
Michelson interference-based self-interferencephase microscopy	2017	Pancreatic		√	Imaging speed:1000/15 h^-1;duration: 1.5 h	Dynamic; cellculture quality
Digital holographicmicroscopy	2017	Jimt-1 cells;SK-MEL-5cells		√	Duration: 36 h	Dynamic; cell proliferation assays
Optical diffractiontomography	2017	Sh-SY5Y cells		√		Dynamic; diagnosis forparkinson's disease
Digital holographicmicroscopy apparatuswith pre-magnification	2012	Osteoblasts and bonecells, paramecia,red cells,cervical cancer cells		√	Duration: 8 h	Dynamic
Laser scanningcytometer	1999	Cells	√		Imaging speed:several hundreds ofcells per second	Dynamic
Parallel microfluidicflow cytometer	2011	Cells	√			Dynamic; diagnosisfor protein-misfoldingdiseases
Microfabricated multiplefield of view imagingflow cytometer	2012	Red blood cells,acute myeloidleukemia cells	√		Imaging speed:2000-20000 s^-1	Dynamic; cellsmorphology andcharacterized
Serial time encodedamplified microscopy	2012	White bloodt-cells; coloncancer cells	√		Imaging speed:100000 s^-1;classificationaccuracy: 95.5%	Dynamic; earlydetection anddiagnosis forblood diseases
Optofluidic timestretch microscopy	2017	Blood cells	√		Imaging speed:100000 s^-1;classificationaccuracy: 96.6%	Dynamic; therapeuticmonitoring forthrombotic
Machine-learning optofluidictime-stretch quantitativephase microscopy	2017	Microalgae cells	√		Imaging speed: 10000 s^-1;classification accuracy: 97%	Dynamic
Common-path withoutmicro-objectivephase microscopy	2015	Blood cells	√		Resolution: <4 μm	Dynamic
Multiwavelength lens-freevideo microscopy	2017	Mesenchymal,endothelial,epithelial cells		√	Duration:several days	Dynamic;dense cells
Digital holographicmicroscopy	2017	Red cells		√		Dynamic; early detectionand diagnosis for blooddiseases

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表 2相位恢复方法的特点

Table2. Characteristics of phase retrieval methods

Technology	Phase shift steps	One shot	Status of sample
Coaxial interference phase shift	≥3	×	Static
Phase extraction in wavelength tuning interferometry	≥3	×	Static
Off-axis interference phase shift	2	√	Static
Fourier transform	1	√	Static
Hilbert transform	1	√	Static
Gram-Schmidt orthonormalization and improvedGram-Schmidt orthonormalization	2	√	Dynamic
Interference fringe differentiation	1	√	Dynamic
New image segmentation	≥1	√	Dynamic

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表 3静态生物细胞相位测量技术的主要特征

Table3. Characteristics of phase measurement methods for static biological cells

Technology	Year	Coaxial	Off-axis	Steps ofphase shift	Digitalholographic	Characteristic	Imagingdimension
Fourier phasemicroscopy	2004	√		4		Sampling rate:4 frame·min^-1	2D
Fast-Fourier phasemicroscopy	2007	√		4		Lateral resolution:diffraction limit;vertical resolution: 2 nm;sampling rate≥10 frame·s^-1	2D
Space light interferencemicroscopy	2011	√		4		Resolution: the same asatomic force microscope;sampling rate≥10 frame·s^-1	2D
White light-Fourierphase microscopy	2013	√		4		Sampling rate: 12.5 frame·s^-1	2D
Parallel two-step phaseshift microscopy	2010	√		2			2D
Full-field opticaltomography technology	2010	√		4			3D
Fresnel diffractionnumerical representation	2005		√		√	Vertical resolution:sub-wavelength	2D
Diffraction phasemicroscopy	2006		√		√		2D
White light-diffractionphase microscopy	2013		√		√	Sampling rate: ms	2D
Non-diffractionreconstruction	2006		√		√	Vertical resolution: 5 nm;sampling rate: ms	2D
Digital holographicmicroscopy based onMichelson interference	2011		√		√	Lateral resolution:sub-cellular	2D
Off-axis interferenceasynchronousdigital holography	2007		√	2	√	Sampling rate: ms	2D
Dual channelinterference microscopy	2009		√	2	√	Sampling rate: ms	3D

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张璐, 赵春晖, 康森柏, 赵宏, 张春伟, 袁莉. 生物细胞定量相位测量与恢复方法研究进展[J]. 中国激光, 2018, 45(2): 0207009. Zhang Lu, Zhao Chunhui, Kang Senbai, Zhao Hong, Zhang Chunwei, Yuan Li. Progress on Methods of Quantitative Phase Measurement and Retrieval for Biological Cells[J]. Chinese Journal of Lasers, 2018, 45(2): 0207009.

生物细胞定量相位测量与恢复方法研究进展下载： 1510次特邀综述

图 1. 同步相移剪切干涉光路示意图[3-4]

Fig. 1. Experimental setup of simultaneous phase shift shear interference[3-4]

图 2. 离轴数字全息光路示意图。(a)文献[ 10];(b)文献[ 12];(c)文献[ 13];(d)文献[ 15];(e)文献[ 16]

Fig. 2. Schematic of off-axis digital holographic. (a) Ref. [10]; (b) Ref. [12]; (c) Ref. [13]; (d) Ref. [15]; (e) Ref. [16]

图 3. 基于流体聚焦技术的光路示意图[44]

Fig. 3. Experimental setup of optofluidic time-stretch quantitative phase microscope[44]

图 4. (a)免显微透镜的共光路相位显微装置[48];(b)多波长免透镜成像显微装置[49];(c)数字全息与全息光镊相结合的光路示意图[50]

Fig. 4. (a) Experimental setup of phase microscopy imaging based on common-path without micro-objective[48];(b) multi-wavelength lens-free video microscopy[49]; (c) schematic of the combined DHM and HOT workstation using holographic optical stretching and quantitative phase imaging of RBCs[50]

表 1动态生物细胞相位测量技术

Table1. Phase measurement methods for dynamic biological cells

表 2相位恢复方法的特点

Table2. Characteristics of phase retrieval methods

表 3静态生物细胞相位测量技术的主要特征

Table3. Characteristics of phase measurement methods for static biological cells

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图 1. 同步相移剪切干涉光路示意图[3-4]

Fig. 1. Experimental setup of simultaneous phase shift shear interference[3-4]

图 2. 离轴数字全息光路示意图。(a)文献[ 10];(b)文献[ 12];(c)文献[ 13];(d)文献[ 15];(e)文献[ 16]

Fig. 2. Schematic of off-axis digital holographic. (a) Ref. [10]; (b) Ref. [12]; (c) Ref. [13]; (d) Ref. [15]; (e) Ref. [16]

图 3. 基于流体聚焦技术的光路示意图[44]

Fig. 3. Experimental setup of optofluidic time-stretch quantitative phase microscope[44]

图 4. (a)免显微透镜的共光路相位显微装置[48];(b)多波长免透镜成像显微装置[49];(c)数字全息与全息光镊相结合的光路示意图[50]

Fig. 4. (a) Experimental setup of phase microscopy imaging based on common-path without micro-objective[48];(b) multi-wavelength lens-free video microscopy[49]; (c) schematic of the combined DHM and HOT workstation using holographic optical stretching and quantitative phase imaging of RBCs[50]

表 1动态生物细胞相位测量技术

Table1. Phase measurement methods for dynamic biological cells

表 2相位恢复方法的特点

Table2. Characteristics of phase retrieval methods

表 3静态生物细胞相位测量技术的主要特征

Table3. Characteristics of phase measurement methods for static biological cells

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生物细胞定量相位测量与恢复方法研究进展下载： 1510次特邀综述