光学学报, 2018, 38 (3): 0318001, 网络出版: 2018-03-20
基于金属光栅近场衍射增强的结构光照明技术 
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Near-Field Diffraction Enhanced Structured Illumination Microscopy Based on Metallic Grating
图 & 表
图 1. (a)近场衍射增强结构光照明技术的原理示意图;(b)金属光栅的结构;(c)光束穿过物镜后波矢和偏振方向的变化
Fig. 1. (a) Principle diagram of near-field diffraction enhanced structure illumination microscopy; (b) structure of metal grating; (c) changes of wave vector and polarization after a light beam pass through the objective
图 2. (a) kx=kg、ky=0、Δφ=0条件下金属光栅激励起的照明条纹光强分布;(b)照明条纹的傅里叶空间级数分布;(c)有限元方法计算得到的金属光栅的近场光强分布
Fig. 2. (a) Spatial intensity distribution of illumination stripe excited by metallic grating under the condition of kx=kg, ky=0, and Δφ=0; (b) Fourier spatial order of illumination stripe; (c) near-field intensity distribution of metallic grating calculated by FEM
图 3. NDESIM技术的亚波长成像性能。(a)经典宽场照明下的点扩展函数;(b) NDESIM的点扩展函数;(c) x轴上的光强分布;(d)宽场照明下的光学传递函数;(e) NDESIM的光学传递函数;(f)被观测物的空间分布;(g)宽场照明的像空间分布;(h) NDESIM的像空间分布;(i)宽场显微和NDESIM成像性能对比
Fig. 3. Sub-wavelength imaging performance of NDESIM. (a) Point spread function (PSF) of classical wide-field imaging; (b) PSF of NDESIM; (c) normalized intensity distribution on the x axis; (d) optical transfer function (OTF) of wide-field imaging; (e) OTF of NDESIM; (f) spatial distribution of object; (g) spatial distribution of wide-field image; (h) spatial distribution of NDESIM image; (i) imaging capability of wide-field imaging and NDESIM
图 4. 金属光栅周期为240 nm时NDESIM技术的亚波长成像性能。(a)照明条纹的傅里叶级数分布;(b) NDESIM的点扩展函数;(c) NDESIM的光学调制传递函数
Fig. 4. Sub-wavelength imaging performance of NDESIM with period of metal grating of 240 nm. (a) Normalized Fourier order of illumination stripe; (b) point spread function of NDESIM; (c) optical modulation transfer function of NDESIM
图 5. NDESIM技术的二维亚波长成像性能。(a)最大后验概率评估算法直接得到的点扩散函数;(b) Lukosz频率滤波后的点扩散函数;(c)光学调制传递函数;(d)沿x轴切线上的光强分布对比
Fig. 5. 2-D sub-wavelength imaging capability of NDESIM. (a) Directed solved point spread function using MAP estimation method; (b) point spread function after Lukosz filtering; (c) optical modulation transfer function; (d) normalized intensity distributions along x-axis
余慕欣, 周文超, 周松, 吴一辉. 基于金属光栅近场衍射增强的结构光照明技术[J]. 光学学报, 2018, 38(3): 0318001. Yu Muxin, Zhou Wenchao, Zhou Song, Wu Yihui. Near-Field Diffraction Enhanced Structured Illumination Microscopy Based on Metallic Grating[J]. Acta Optica Sinica, 2018, 38(3): 0318001.
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