Chinese Optics Letters, 2019, 17 (11): 110901, Published Online: Sep. 12, 2019  

Pixel super-resolution lensless in-line holographic microscope with hologram segmentation Download: 835次

Author Affiliations
Biophotonics Laboratory, University of Michigan—Shanghai Jiao Tong University Joint Institute, Shanghai Jiao Tong University, Shanghai 200240, China
Figures & Tables

Fig. 1. (a) Schematic of the LIHM system setup; (b) photograph of the system.

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Fig. 2. Flowchart of the reconstruction process in our method.

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Fig. 3. Numerical simulation to demonstrate our method with a two-layer sample. (a) The top layer of the sample; (b) the bottom layer of the sample; (c) one of the in-line holograms of the two-layer sample; (d) the segmented hologram of the USAF target elements obtained from (c); (e) the holographic reconstruction result according to (c); (f) PSR reconstruction using multiple low-resolution images of (e); (g) PSR reconstruction using multiple segmented low-resolution images obtained from (d).

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Fig. 4. Experiment with a two-layer sample, where the top layer is a USAF target, and the bottom layer contains aggregations of microspheres. (a) The schematic of the two-layer sample; (b) one of the in-line holograms of the two-layer sample; (c) the conventional holographic reconstruction result according to (b); (d) the enlarged image of the region indicated in (c); (e) PSR reconstruction using multiple low-resolution images of (d); (f) PSR reconstruction using multiple low-resolution images acquired by segmentation and reconstruction of the enlarged region shown in (d).

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Fig. 5. Experiments with the sample of water solution containing the algae Euglena gracilis. (a) One of the in-line holograms; (b) the conventional holographic reconstruction according to (a), where the target alga and some adjacent objects can be observed; (c) the segmented in-line hologram of the target alga as indicated in (a); (d) the enlarged image of the target alga as indicated in (b); (e) PSR reconstruction of the target alga without using the segmentation technique; (f) PSR reconstruction of the target alga with applying the segmentation technique; (g) microscope image of similar alga observed under a 20× objective.

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Fig. 6. Atlas of algae images obtained by PSR reconstruction after the segmentation technique in our LIHM system.

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Mingjun Wang, Shaodong Feng, Jigang Wu. Pixel super-resolution lensless in-line holographic microscope with hologram segmentation[J]. Chinese Optics Letters, 2019, 17(11): 110901.

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