Journal of Innovative Optical Health Sciences, 2019, 12 (4): 1942003, Published Online: Sep. 3, 2019  

Multidither coherent optical adaptive technique for deep tissue two-photon microscopy

Author Affiliations
1 State Key Laboratory of Modern Optical Instrumentation, Department of Neurobiology of the First A±liated Hospital, Zhejiang University School of Medicine, Hangzhou 310027, P. R. China
2 College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. China
3 NHC and CAMS Key Laboratory of Medical Neurobiology, Department of Neurobiology, Center for Neuroscience, Zhejiang University School of Medicine, Hangzhou 310058, P. R. China
Abstract
Two-photon microscopy normally suffers from the scattering of the tissue in biological imaging. Multidither coherent optical adaptive technique (COAT) can correct the scattered wavefront in parallel. However, the determination of the corrective phases may not be completely accurate using conventional method, which undermines the performance of this technique. In this paper, we theoretically demonstrate a method that can obtain more accurate corrective phases by determining the phase values from the square root of the fluorescence signal. A numerical simulation model is established to study the performance of adaptive optics in two-photon microscopy by combining scalar diffraction theory with vector diffraction theory. The results show that the distortion of the wavefront can be corrected more thoroughly with our method in two-photon imaging. In our simulation, with the scattering from a 450-μm-thick mouse brain tissue, excitation focal spots with higher peak-to-background ratio (PBR) and images with higher contrast can be obtained. Hence, further enhancement of the multidither COAT correction performance in two-photon imaging can be expected.
References

[1] N. Ji et al., “Advances in the speed and resolution of light microscopy," Curr. Opin. Neurobiol. 18(6), 605–616 (2008).

[2] F. Helmchen, W. Denk, “Deep tissue two-photon microscopy," Nat. Methods 2(12), 932 (2005).

[3] S. Gigan, “Optical microscopy aims deep," Nat Photonics 11(1), 14–16 (2017).

[4] J. A. Kubby, Adaptive Optics for Biological Imaging, 1st Edition (CRC Press, Boca Raton, 2013).

[5] M. J. Booth, “Adaptive optical microscopy: The ongoing quest for a perfect image," Light Sci. Appl. 3(4), e165 (2014).

[6] D. D. Battista, G. Zacharakis, M. Leonetti, “Enhanced adaptive focusing through semi-transparent media," Sci. Rep. 5, 17406 (2015).

[7] W. Zheng et al., “Adaptive optics improves multiphoton super-resolution imaging," Nat. Methods 14, 869–872 (2017).

[8] D. Champelovier et al., “Image-based adaptive optics for in vivo imaging in the hippocampus," Sci. Rep. 7, 42924 (2017).

[9] R. K. Tyson, Principles of Adaptive Optics, 4th Edition (CRC Press, Boca Raton, 2016).

[10] M. J. Booth, “Adaptive optics in microscopy," Philos. Trans. A Math. Phys. Eng. Sci. 365(1861), 2829–2843 (2007).

[11] M. A. A. Neil et al., “Adaptive aberration correction in a two-photon microscope," J. Microsc. 200(2), 4 (2000).

[12] M. J. Booth et al., “Adaptive aberration correction in a confocal microscope," Proc. Natl. Acad. Sci. 99(9), 5788–5792 (2002).

[13] M. J. Booth, M. Neil, T. Wilson, “New modal wavefront sensor: Application to adaptive confocal fluorescence microscopy and two-photon excitation fluorescence microscopy," J. Opt. Soc. Am. A 19(10), 2112–2120 (2002).

[14] M. J. Booth, “Wavefront sensorless adaptive optics for large aberrations," Opt. Lett. 32(1), 5 (2007).

[15] D. Debarre, M. J. Booth, T. Wilson, “Image based adaptive optics through optimisation of low spatial frequencies," Opt. Express 15(13), 8176–8190 (2007).

[16] D. Debarre et al., “Adaptive optics for structured illumination microscopy," Opt. Express 16(13), 9290 (2008).

[17] D. Debarre et al., “Image-based adaptive optics for two-photon microscopy," Opt. Lett. 34(16), 2495–2497 (2009).

[18] M. Yamanaka et al., “Optical coherence microscopy in 1700 nm spectral band for high-resolution labelfree deep-tissue imaging," Sci. Rep. 6, 31715 (2016).

[19] J. W. Hardy, L. Thompson, “Adaptive optics for astronomical telescopes," Phys. Today 53(4), 69 (2000).

[20] E. J. Fernandez et al., “Adaptive optics with a magnetic deformable mirror: Applications in the human eye," Opt. Express 14(20), 8900 (2006).

[21] A. Roorda et al., “Adaptive optics scanning laser ophthalmoscopy," Opt. Express 10(9), 405–12 (2002).

[22] X. Tao et al., “Adaptive optics microscopy with direct wavefront sensing using fluorescent protein guide stars," Opt. Lett. 36(17), 3389–91 (2011).

[23] B. R. Masters, “Adaptive optics for biological imaging," J. Biomed. Opt. 18(7), 1–2 (2013).

[24] M. Cui, C. Yang, “Implementation of a digital optical phase conjugation system and its application to study the robustness of turbidity suppression by phase conjugation," Opt. Express 18(4), 3444–3455 (2010).

[25] C. L. Hsieh et al., “Digital phase conjugation of second harmonic radiation emitted by nanoparticles in turbid media," Opt. Express 18(12), 12283–12290 (2010).

[26] N. Ji, D. E. Milkie, E. Betzig, “Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues," Nat. Methods 7(2), 141 (2010).

[27] J. Liang, D. R. Williams, D. T. Miller, “Supernormal vision and high-resolution retinal imaging through adaptive optics," J. Opt. Soc. Am. A 14(11), 2884–2892 (1997).

[28] M. Rueckel, J. A. Mackbucher, W. Denk, “Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing," Proc. Natl. Acad. Sci. USA 103(46), 17137–17142 (2006).

[29] O. Albert et al., “Smart microscope: An adaptive optics learning system for aberration correction in multiphoton confocal microscopy," Opt. Lett. 25(1), 52–54 (2000).

[30] P. Marsh, D. Burns, J. Girkin, “Practical implementation of adaptive optics in multiphoton microscopy," Opt. Express 11(10), 1123–30 (2003).

[31] L. Sherman et al., “Adaptive correction of depthinduced aberrations in multiphoton scanning microscopy using a deformable mirror," J. Microsc. 206(1), 65–71 (2010).

[32] A. J. Wright et al., “Exploration of the optimisation algorithms used in the implementation of adaptive optics in confocal and multiphoton microscopy," Microsc. Res. Tech. 67, 36–44 (2005).

[33] C. Wang, N. Ji, “Characterization and improvement of three-dimensional imaging performance of GRIN-lens-based two-photon fluorescence endomicroscopes with adaptive optics," Opt. Express 21(22), 27142–27154 (2013).

[34] M. Cui, “Parallel wavefront optimization method for focusing light through random scattering media," Opt. Lett. 36(6), 870 (2011).

[35] T. R. O'Meara, “The multidither principle in adaptive optics," J. Opt. Soc. Am. 67 306–315 (1977).

[36] R. Liu et al., “Direct phase measurement in zonal wavefront reconstruction using multidither coherent optical adaptive technique," Opt. Express 22(2), 1619–1628 (2014).

[37] J. J. Stamnes, Waves in Focal Regions: Propagation, Diffraction and Focusing of Light, Sound and Water Waves, Adam Hilger, Bristol (1986).

[38] T. Kozacki, “Numerical errors of diffraction computing using plane wave spectrum decomposition," Opt. Commun. 281, 4219–4223 (2008).

[39] B. Richards, E. Wolf, “Electromagnetic diffraction in optical systems. II. Structure of the image field in an aplanatic system," Proc. A 253(1274), 358–379 (1959).

[40] K. Youngworth, T. Brown, “Focusing of high numerical aperture cylindrical-vector beams," Opt. Express 7(2), 77–87 (2000).

[41] B. Zhu et al., “Numerical studies of focal modulation microscopy in high-NA system," Opt. Express 24(17), 19138–19147 (2016).

Biwei Zhang, Wei Gong, Chenxue Wu, Lejia Hu, Xinpei Zhu, Ke Si. Multidither coherent optical adaptive technique for deep tissue two-photon microscopy[J]. Journal of Innovative Optical Health Sciences, 2019, 12(4): 1942003.

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