Preparation of two-photon fluorescent probe and biological imaging application in cells

Shuheng Chi; Liang Li; Yiqun Wu

doi:doi:10.3788/COL201614.061603

Chinese Optics Letters, 2016, 14 (6): 061603, Published Online: Aug. 6, 2018

Preparation of two-photon fluorescent probe and biological imaging application in cells Download： 783次

Shuheng Chi ¹Liang Li ¹Yiqun Wu ^1,2,*

Author Affiliations

¹ Key Laboratory of Material Science and Technology for High Power Lasers, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China

² Key Lab of Functional Inorganic Material Chemistry (Heilongjiang University), Ministry of Education, Harbin 150080, China

Figures & Tables

Fig. 1. Synthesis of dibenzothiophene-based fluorescent probe.

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Fig. 2. Normalized UV absorption and one-photon fluorescence (FL) spectra of TP and TPI in DMF, [TPI]=10−5 mol/L.

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Fig. 3. Two-photon fluorescence spectra of TPI and DAPI in DMF, [TPI]=[DAPI]=10−4 mol/L.

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Fig. 4. (a) and (c) UV absorption and OPEF responses of DAPI and (b) and (d) TPI upon the titration of DNA in the Tris-HCl buffer solutions, [TPI]=[DAPI]=10−5 mol/L, [DNA]=0−1.3×10−3 mol/L.

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Fig. 5. One-photon and two-photon confocal fluorescence images of 3T3 cells stained with TPI (0.5 μmol/L) for 3 h. (a) TPEF bioimaging; (b) DIC picture; (c) OPEF bioimaging; (d) Merged bioimaging of a and c. The wavelength for two-photon and one-photon excitation was 800 and 405 nm, respectively. Scale bar was 50 μm.

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Fig. 6. Confocal fluorescence images of 3T3 cells co-stained with TPI (0.5 μmol/L) and MTR (0.5 μmol/L). For TPI, λex=405 nm, and λem=525−575 nm. For MTR, λex=575 nm, and λem=585−660 nm. Scale bar was 50 μm.

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Fig. 7. Time-dependent confocal fluorescence bioimaging of 3T3 cells stained with TPI (0.5 μmol/L) for 15 min. The excitation wavelength was 800 nm. Scale bar was 50 μm.

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Fig. 8. Time-dependent confocal fluorescence bioimaging of 3T3 cells stained with DAPI (0.5 μmol/L) for 15 min. The excitation wavelength was 740 nm. Scale bar was 20 μm.

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Fig. 9. Comparison of photostability of TPI and DAPI under successive irradiation.

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Shuheng Chi, Liang Li, Yiqun Wu. Preparation of two-photon fluorescent probe and biological imaging application in cells[J]. Chinese Optics Letters, 2016, 14(6): 061603.

Preparation of two-photon fluorescent probe and biological imaging application in cells Download： 783次

Fig. 1. Synthesis of dibenzothiophene-based fluorescent probe.

Fig. 2. Normalized UV absorption and one-photon fluorescence (FL) spectra of TP and TPI in DMF, [TPI]=10−5 mol/L.

Fig. 3. Two-photon fluorescence spectra of TPI and DAPI in DMF, [TPI]=[DAPI]=10−4 mol/L.

Fig. 4. (a) and (c) UV absorption and OPEF responses of DAPI and (b) and (d) TPI upon the titration of DNA in the Tris-HCl buffer solutions, [TPI]=[DAPI]=10−5 mol/L, [DNA]=0−1.3×10−3 mol/L.

Fig. 6. Confocal fluorescence images of 3T3 cells co-stained with TPI (0.5 μmol/L) and MTR (0.5 μmol/L). For TPI, λex=405 nm, and λem=525−575 nm. For MTR, λex=575 nm, and λem=585−660 nm. Scale bar was 50 μm.

Fig. 7. Time-dependent confocal fluorescence bioimaging of 3T3 cells stained with TPI (0.5 μmol/L) for 15 min. The excitation wavelength was 800 nm. Scale bar was 50 μm.

Fig. 8. Time-dependent confocal fluorescence bioimaging of 3T3 cells stained with DAPI (0.5 μmol/L) for 15 min. The excitation wavelength was 740 nm. Scale bar was 20 μm.

Fig. 9. Comparison of photostability of TPI and DAPI under successive irradiation.

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Preparation of two-photon fluorescent probe and biological imaging application in cells Download： 783次

Fig. 1. Synthesis of dibenzothiophene-based fluorescent probe.

Fig. 2. Normalized UV absorption and one-photon fluorescence (FL) spectra of TP and TPI in DMF, [TPI]=10−5 mol/L.

Fig. 3. Two-photon fluorescence spectra of TPI and DAPI in DMF, [TPI]=[DAPI]=10−4 mol/L.

Fig. 4. (a) and (c) UV absorption and OPEF responses of DAPI and (b) and (d) TPI upon the titration of DNA in the Tris-HCl buffer solutions, [TPI]=[DAPI]=10−5 mol/L, [DNA]=0−1.3×10−3 mol/L.

Fig. 6. Confocal fluorescence images of 3T3 cells co-stained with TPI (0.5 μmol/L) and MTR (0.5 μmol/L). For TPI, λex=405 nm, and λem=525−575 nm. For MTR, λex=575 nm, and λem=585−660 nm. Scale bar was 50 μm.

Fig. 7. Time-dependent confocal fluorescence bioimaging of 3T3 cells stained with TPI (0.5 μmol/L) for 15 min. The excitation wavelength was 800 nm. Scale bar was 50 μm.

Fig. 8. Time-dependent confocal fluorescence bioimaging of 3T3 cells stained with DAPI (0.5 μmol/L) for 15 min. The excitation wavelength was 740 nm. Scale bar was 20 μm.

Fig. 9. Comparison of photostability of TPI and DAPI under successive irradiation.

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