中国激光, 2012, 39 (6): 0615001, 网络出版: 2012-05-04   

维生素B2固体粉末荧光偏振特性及其检测

Fluorescence Polarization Properties and Detection of Vitamin B2 Solid Powder
作者单位
南开大学现代光学研究所, 光学信息技术教育部重点实验室, 天津 300071
摘要
将固体表面荧光法和荧光偏振测量相结合,直接利用固体粉末进行荧光偏振检测,研究了维生素B2固体粉末的荧光偏振度及其特性。实验结果表明,维生素B2固体粉末的荧光偏振度受激发光入射角影响;当样品粉末紧密度大于0.6667时,紧密度不会对荧光偏振度产生影响。在激发光入射角为45°条件下其荧光偏振度为0.4178。该结果为使用维生素B2水溶液进行荧光偏振检测的结果的1.42倍。对维生素B2固体粉末连续进行6 h照射激发,其荧光偏振度检测结果在误差范围内保持稳定。
Abstract
Using solid powder directly, an approach to acquire the fluorescence polarization is demonstrated by combining solid surface fluorimetry and fluorescence polarization, and the fluorescence polarization properties of vitamin B2 are researched with this method. The results show that the degree of fluorescence polarization of vitamin B2 solid powder is dependent on the incident angle of the exciting light, and is independent of the relative compactness when it is greater than 0.6667. The degree of polarization hits 0.4178 while the incident angle is 45°. It is 1.42 times of that the detected result from the aqueous solution. The degree of fluorescence polarization of the vitamin B2 solid sample keeps steadily in error band under constant illumination for 6 h.
参考文献

[1] J. B. F. Lloyd. Synchronized excitation of fluorescence emission spectra[J]. Nature Physical Science, 1971, 231(5): 64~65

[2] A. Warsinke, A. Benkert, F. W. Scheller. Electrochemical immunoassays[J]. Fresenius J. Anal. Chem., 2000, 366(6-7): 622~634

[3] M. S. Wrighton, D. S. Giniey, D. L. Morse. A technique for determination of absolute emission quantum yields of powdered samples[J]. J. Phys. Chem., 1974, 78(22): 2229~2233

[4] 杨仁杰, 徐晓轩, 尚丽平 等. 含油岩屑固体粉末荧光的研究[J]. 发光学报, 2005, 26(1): 131~134

    Yang Renjie, Xu Xiaoxuan, Shang Liping et al.. Solid powder fluorescence of oil cuttings[J]. Chinese J. Luminescence, 2005, 26(1): 131~134

[5] 张春艳, 张伟刚, 王超 等. 含油岩芯固体粉末荧光光谱的直接检测[J]. 中国激光, 2009, 36(4): 918~922

    Zhang Chunyan, Zhang Weigang, Wang Chao et al.. Direct inspecting of solid powder fluorescence of oil cuttings[J]. Chinese J. Lasers, 2009, 36(4): 918~922

[6] 彭雪松, 张伟刚, 张春艳 等. 固态面粉中维生素B6的荧光法直接测定[J]. 光电子·激光, 2011, 22(4): 566~569

    Peng Xuesong, Zhang Weigang, Zhang Chunyan et al.. Fluorescent determination of vitamin B6 in solid flour[J]. Journal of Optoelectronics·Laser, 2011, 22(4): 566~569

[7] 刘春阳, 孙立东, 傅星 等. 355 nm纳秒脉冲激光在硅表面照射形成微结构及其荧光检测[J]. 中国激光, 2010, 37(8): 2139~2142

    Liu Chunyang, Sun Lidong, Fu Xing et al.. Si microstructure fabricated by 355 nm nanosecond pulsed laser and its fluorescence microscopy study[J]. Chinese J. Lasers, 2010, 37(8): 2139~2142

[8] 李子雷, 王琳, 庞其昌 等. 基于液晶光谱仪的西洋参品质荧光检测方法[J]. 光学学报, 2010, 30(12): 3622~3626

    Li Zilei, Wang Lin, Pang Qichang et al.. Panax quinquefolium′s active ingredients detecting method based on LCTF spectrograph[J]. Acta Optica Sinica, 2010, 30(12): 3622~3626

[9] 庄任重, 赖国忠, 林福忠 等. 掺铒钼酸钙激光晶体的生长与光谱研究[J]. 中国激光, 2010, 37(12): 3108~3111

    Zhuang Renzhong, Lai Guozhong, Lin Fuzhong et al.. Growth and spectral properties of calcium molybdate laser crystal doped with erbium ion[J]. Chinese J. Lasers, 2010, 37(12): 3108~3111

[10] U. Utzinger, R. R. Richards-Kortum. Fiber optic probes for biomedical optical spectroscopy[J]. J. Biomed. Opt., 2003, 8(1): 121~147

[11] A. E. Erolu, M. Volkan, O. Y. Ataman. Fiber optic sensors using novel substrates for hydrogen sulfide determination by solid surface fluorescence[J]. Talanta, 2000, 53(1): 89~101

[12] 王玉田, 胡俏丽, 石军彦. 基于荧光机理的光纤温度测量仪[J]. 光学学报, 2010, 30(3): 655~659

    Wang Yutian, Hu Qiaoli, Shi Junyan. Optical fiber thermometer based on fluorescence mechanism[J]. Acta Optica Sinica, 2010, 30(3): 655~659

[13] F. Weigert, G. Kppler. Polarisierte fluoreszenz in farbstofflsungen[J]. Zeitschrift für Physik A Hadrons and Nuclei,1925, 25(1): 99~117

[14] F. Perrin. Polarization de la lumière de fluorescence. vie moyenne des mloécules dans l′etat excite[J]. J. Phys. Radium, 1926, 7(12): 390~401

[15] G. Weber. Polarization of the fluorescence of macromolecules[J]. Biochemical Journal, 1952, 51(2): 145~167

[16] 彭长德, 刘莹, 兰秀风 等. 血液静态荧光偏振光谱研究[J]. 光学学报, 2006, 26(10): 1543~1548

    Peng Changde, Liu Ying, Lan Xiufeng et al.. Research on static fluorescence polarization spectrum of whole blood[J]. Acta Optica Sinica, 2006, 26(10): 1543~1548

[17] 刘莹, 何文亮, 刘诚. 乙醇中毒血液荧光偏振特性研究[J]. 光学学报, 2010, 30(7): 2065~2068

    Liu Ying, He Wenliang, Liu Cheng. Research on the fluorescence polarization characteristic of alcoholism blood[J]. Acta Optica Sinica, 2010, 30(7): 2065~2068

[18] 张天泰, 杜冠华. 极化荧光在高通量药物筛选中的应用[J]. 药学学报, 2005, 40(4): 289~293

    Zhang Tiantai, Du Guanhua. Application of fluorescence polarization-based assay in high throughput screening[J]. Acta Pharmaceutica Sinica, 2005, 40(4): 289~293

[19] 王金华, 韩光亮, 张天泰 等. 基于FP法的EPOR配体高通量筛选模型[J]. 中国药理学通报, 2006, 22(1): 114~117

    Wang Jinhua, Han Guangliang, Zhang Tiantai et al.. A high-throughput fluorescent polarization model for screening EPO receptor ligand[J]. Chinese Pharmacological Bulletin, 2006, 22(1): 114~117

[20] M. Zandomeneghi. Fluorescence of cereal flours[J]. J. Agric. Food Chem., 1999, 47(3): 878~882

[21] T. L. Mann, U. J. Krull. Fluorescence polarization spectroscopy in protein analysis[J]. Analyst, 2003, 128(4): 313~317

[22] D. M. Jameson, J. A. Ross. Fluorescence polarization/anisotropy in diagnostics and imaging[J]. Chem. Rev., 2010, 110(5): 2685~2708

[23] A. B. Moreira, H. P. M. Oliveira, T. D. Z. Atvars et al.. Direct determination of paracetamol in powdered pharmaceutical samples by fluorescence spectroscopy[J]. Analytica Chimica Acta, 2005, 539(1-2): 257~261

[24] M. Zandomeneghi, L. Carbonaro, L. Calucci et al.. Direct fluorometric determination of fluorescent substances in powders: the case of riboflavin in cereal flours[J]. J. Agric. Food Chem., 2003, 51(10): 2888~2895

严肃源, 张伟刚. 维生素B2固体粉末荧光偏振特性及其检测[J]. 中国激光, 2012, 39(6): 0615001. Yan Suyuan, Zhang Weigang. Fluorescence Polarization Properties and Detection of Vitamin B2 Solid Powder[J]. Chinese Journal of Lasers, 2012, 39(6): 0615001.

本文已被 2 篇论文引用
被引统计数据来源于中国光学期刊网
引用该论文: TXT   |   EndNote

相关论文

加载中...

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!