H<sub>2</sub>O<sub>2</sub> INDUCES APOPTOSIS OF RABBIT CHONDROCYTES VIA BOTH THE EXTRINSIC AND THE CASPASE-INDEPENDENT INTRINSIC PATHWAYS

CAIPING ZHUANG; XIAOPING WANG; TONGSHENG CHEN

doi:doi:10.1142/s1793545813500223

Journal of Innovative Optical Health Sciences, 2013, 6 (3): 1350022, Published Online: Jan. 10, 2019

H₂O₂ INDUCES APOPTOSIS OF RABBIT CHONDROCYTES VIA BOTH THE EXTRINSIC AND THE CASPASE-INDEPENDENT INTRINSIC PATHWAYS

论文大纲

CAIPING ZHUANG ¹XIAOPING WANG ^1,*TONGSHENG CHEN ²

Author Affiliations

¹ Department of Pain Management The First Affiliated Hospital of Jinan University Guangzhou 510632, P. R. China

² MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science South China Normal University Guangzhou 510631, P. R. China

Chondrocytes apoptosis H₂O₂ caspases AIF

Abstract

Osteoarthritis (OA), one of the most common joint diseases with unknown etiology, is characterized by the progressive destruction of articular cartilage and the apoptosis of chondrocytes. The purpose of this study is to elucidate the molecular mechanisms of H₂O₂-mediated rabbit chondrocytes apoptosis. CCK-8 assay showed that H₂O₂ treatment induced a remarkable reduction of cell viability, which was further verified by the remarkable phosphatidylserine externalization after H₂O₂ treatment for 1 h, the typical characteristics of apoptosis. H₂O₂ treatment induced a significant dysfunction of mitochondrial membrane potential (ΔΨm), but did not induce casapse- 9 activation, indicating that H₂O₂ treatment induced caspase-independent intrinsic apoptosis that was further verified by the fact that silencing of AIF but not inhibiting caspase-9 potently prevented H₂O₂-induced apoptosis. H₂O₂ treatment induced a significant increase of caspase-8 and -3 activation, and inhibition of caspase-8 or -3 significantly prevented H₂O₂-induced apoptosis, suggesting that the extrinsic pathway played an important role. Collectively, our findings demonstrate that H₂O₂ induces apoptosis via both the casapse-8-mediated extrinsic and the caspase-independent intrinsic apoptosis pathways in rabbit chondrocytes.

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CAIPING ZHUANG, XIAOPING WANG, TONGSHENG CHEN. H₂O₂ INDUCES APOPTOSIS OF RABBIT CHONDROCYTES VIA BOTH THE EXTRINSIC AND THE CASPASE-INDEPENDENT INTRINSIC PATHWAYS[J]. Journal of Innovative Optical Health Sciences, 2013, 6(3): 1350022.

H₂O₂ INDUCES APOPTOSIS OF RABBIT CHONDROCYTES VIA BOTH THE EXTRINSIC AND THE CASPASE-INDEPENDENT INTRINSIC PATHWAYS

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