应用光镊拉曼光谱系统俘获单个红细胞并收集其拉曼光谱,选择I1638/I1547比值为区分红细胞氧合及去氧态指标,比较分析地中海贫血HbH-CS和健康个体单个红细胞的氧循环过程的氧合和去氧能力及去氧态群体红细胞。结果发现,在氧循环的去氧态中,地中海贫血HbH-CS 的I1638/I1547显著高于正常对照;氧合态的I1638/I1547也略高于正常对照。群体统计中,若以小于正常对照I1638/I1547的平均值为去氧态基准,则正常对照46%的红细胞达到去氧态,而地中海贫血HbH-CS仅有15 %,表明地中海贫血HbH-CS红细胞的氧合能力较强,但去氧能力显著低于正常对照,而且地中海贫血HbH-CS红细胞个体差异较大。该研究在单细胞水平上,揭示了地中海贫血HbH-CS红细胞氧合、去氧能力以及细胞个体差异性,以评价其携氧功能,为诊断治疗提供一定的理论依据。

A laser tweezers Raman spectroscopy (LTRS) system was used to investigate the capacity of carrying oxygen in thalassemia erythrocytes. Single erythrocyte from patients with thalassemia HbH-CS and normal donors were trapped,and the Raman scattering signal of trapped cell was collected. The intensity ratio of band 1638 cm-1 to band 1547 cm-1 (I1638/I1547) was selected to distinguish oxygenated erythrocytes from deoxygenated ones. By comparing the oxygenation cycle of optically trapped single red blood cells and the deoxygenated cells population from the two kinds of specimen,and analyzing the oxygenated and deoxygenated states,it is found that,in the oxygenation cycle,the ratio I1638/I1547 of thalassemia HbH-CS was much higher than that of normal control in deoxygenated state and slightly higher than the normal control in oxygenated state. In the population statistic,46% of the red blood cells from the normal control reach oxygenated state,but thalassemia HbH-CS only 15%,if the base is set by less than the average of I1638/I1547 from normal control cells. The result shows that the red blood cells of thalassemia HbH-CS are easy to oxygenate,but difficult to deoxygenate. The individual difference between thalassemia HbH-CS erythrocytes is obvious. This study provides the spectral vision on the oxygenation of thalassemia HbH-CS erythrocytes and the laboratorial referrences for the diagnosis and treatment.