光学学报, 1988, 8 (8): 759, 网络出版: 2011-09-16
低浓度O6甲基鸟嘌呤荧光检测的研究
Fluorescence spectrophotometric assay of O6methyIguanine in low concentration
摘要
本文报道了一种采用荧光光谱测定O~6甲基鸟嘌呤的方法.该法具有灵敏度高、选择性好等优点、检测灵灵敏度巳达到0.01fM/ml,比国内外常用的放射免疫法高一个量级.
Abstract
The determination of chemical oarcinogen-DNA adducts is necessary for research of molecular epidemiology and mechanism of chemical carcinogenesis. A method ig presented in this paper for the detection of putative addnots by using fluorescence spectroscopy. The main advantages include high sensitivity and high selectivity. The minimum detectable amount of O6-MEG is 0.01 fmol/ml. The sensitivity of detection is an order of magnitude higher than that of radioimmunoassay.
参考文献
荘大奎, 刘亚淑, 瞿永华, 吴一迁. 低浓度O6甲基鸟嘌呤荧光检测的研究[J]. 光学学报, 1988, 8(8): 759. ZHUANG DAKUI, LIU YASHU, QU YONGHUA, WU YIQIAN. Fluorescence spectrophotometric assay of O6methyIguanine in low concentration[J]. Acta Optica Sinica, 1988, 8(8): 759.
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