光学学报, 2020, 40 (18): 1806001, 网络出版: 2020-08-27
氧化石墨烯包覆金纳米壳修饰长周期光栅的免疫传感器 
下载： 1109次
An Immunosensor Based on the Graphene-Oxide-Encapsulated Au-Nanoshell-Coated Long-Period Fiber Grating
图 & 表
图 2. EGO-AuNS-LPFG免疫传感器制作过程。(a)表面预处理;(b)羟基化;(c)硅烷化;(d)修饰EGO-AuNS;(e)活化羧基基团;(f)传感器表面修饰AIV-MAbs及封闭多余结合位点;(g)AIV抗原检测
Fig. 2. Fabrication of the EGO-AuNS-LPFG immunosensor. (a) Surface pre-treatment; (b) hydroxylation; (c) silylation; (d) modification with EGO-AuNS; (e) activation of carboxyl group; (f) surface modification with AIV-MAbs and sealing of redundant binding sites; (g) detection for AIV antigens
图 4. EGO-AuNS-LPFG的FESEM图像。(a) 5000×;(b) 10000×;(c) 50000×
Fig. 4. FESEM graphs of the EGO-AuNS-LPFG images. (a) 5000×; (b) 10000×; (c) 50000×
图 7. LPFG修饰EGO-AuNS前后光谱随蔗糖溶液RI变化关系。(a)修饰前;(b)修饰后
Fig. 7. Transmission spectra of the bare LPFG and the EGO-AuNS coated LPFG immersed in different sucrose concentrations. (a) Bare LPFG; (b) EGO-AuNS coated LPFG
图 8. 修饰EGO-AuNS前后LPFG的波长RI灵敏度变化
Fig. 8. Resonant wavelength of the bare LPFG and EGO-AuNS coated LPFG against RI of different sucrose solutions
图 9. AIV免疫检测过程中传感器光谱变化和相应谐振波长漂移量随时间的变化。(a)光谱变化;(b)相应谐振波长漂移量
Fig. 9. Spectral evolution and the corresponding wavelength shift of the immunosensor for AIV detection against time. (a) Spectral evolution;(b)corresponding wavelength shift
图 10. 波长漂移量绝对值与抗原浓度关系拟合曲线(插图为低浓度线性拟合区)
Fig. 10. Wavelength shift of the GO-AuNS-LPFG immunosensor for different AIV concentration levels (Inset corresponds to the linear fitting area)
表 1EGO-AuNS-LPFG表面元素成分
Table1. Corresponding percentage content for the surface of the EGO-AuNS-LPFG%
|
石胜辉, 吴德操, 王鑫, 聂青林, 刘志江, 罗彬彬, 刘恩华, 柳鹏, 赵明富. 氧化石墨烯包覆金纳米壳修饰长周期光栅的免疫传感器[J]. 光学学报, 2020, 40(18): 1806001. Shenghui Shi, Decao Wu, Xin Wang, Qinglin Nie, Zhijiang Liu, Binbin Luo, Enhua Liu, Peng Liu, Mingfu Zhao. An Immunosensor Based on the Graphene-Oxide-Encapsulated Au-Nanoshell-Coated Long-Period Fiber Grating[J]. Acta Optica Sinica, 2020, 40(18): 1806001.
PDF全文
