一种高性能金标条阅读仪的研制

陈贝特; 黄立华; 郭凯; 黄惠杰

doi:doi:10.3788/cjl201340.0704001

中国激光, 2013, 40 (7): 0704001, 网络出版: 2013-06-08

一种高性能金标条阅读仪的研制

Development of a High Performance Reader for Colloidal Gold Lateral Flow Strip

论文大纲

陈贝特 ^1,2,*黄立华 ¹郭凯 ¹黄惠杰 ¹

作者单位

¹ 中国科学院上海光学精密机械研究所信息光学与光电技术实验室, 上海 201800

² 中国科学院大学, 北京 100049

摘要

为使金标免疫层析技术能对目标被检物进行高灵敏度快速定量检测，研制了一种基于CMOS图像传感器的金标条阅读仪。根据纳米金颗粒对绿光的强烈吸收特性,使用绿光LED为照明光源；依据空白金标试纸条图像的灰度分布调节LED光强和位置，使成像区域的照明均匀性达到最佳；依据自定义评价参数调节CMOS图像传感器的成像控制参数，使图像质量达到最佳；使用空白金标试纸条图像数据做背景校正，再通过特定算法计算出样品中目标被检物的浓度。使用该金标条阅读仪对滴加有心肌肌钙蛋白I(cTnI)标准样品的金标试纸条进行检测，在质量浓度为0.25～64 ng/mL范围内具有良好的4-PL响应特性,相关系数R2>0.99；使用该金标条阅读仪对上述浓度范围的金标试纸条各进行20次重复测量,其中，对高浓度金标试纸条（64 ng/mL）测量结果的变异系数为0.134%，对低浓度金标试纸条（0.25 ng/mL）测量结果的变异系数为2.790%；功能灵敏度优于0.25 ng/mL。该金标条阅读仪具有重复性好、灵敏、快速、低功耗、小型化等特点。

Abstract

A colloidal gold lateral flow (LF) strip reader based on CMOS image sensor, characterized by ultrasensitive and high repeatable, is developed. As gold nano-particles absorb green light strongly, green LEDs are used as the light source in the reader. In order to get the best illumination uniformity, gray value distribution of blank strip image is used to adjust the location and intensity of LEDs. CMOS control parameters are specified to get the most suitable image quality for image analyzing by self-defining parameters. Blank strip is used to calibrate the background signal. The quantitative result is derived from the ratio of integral reflection optical density of T line signal to C line signal. LF strips of cardiac troponin I (cTnI) with standard mass concentration gradients from 0.25 ng/ml to 64 ng/mL are detected. The 4-PL quantitative model is used to fitting standard working curve, whose correlation of coefficient (R2) is more than 0.99. The coefficient of variation (CV) of 20 times repetitive experiments of 0.25 ng/mL is 2.790%, and 64 ng/mL is 0.134%. The functional sensitivity (FS) is better than 0.25 ng/mL. The reader is sensitive, stable, simple, and suitable for field detection.

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陈贝特, 黄立华, 郭凯, 黄惠杰. 一种高性能金标条阅读仪的研制[J]. 中国激光, 2013, 40(7): 0704001. Chen Beite, Huang Lihua, Guo Kai, Huang Huijie. Development of a High Performance Reader for Colloidal Gold Lateral Flow Strip[J]. Chinese Journal of Lasers, 2013, 40(7): 0704001.

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