近几十年来，光片荧光显微镜作为荧光显微技术的一种革新，显著提升了生命科学研究中对组织与细胞结构和功能的高时空分辨率成像能力。相较于传统的落射荧光显微技术，光片显微镜通过选择性逐层照明生物样本，大大提高了光子利用效率，降低了光毒性，并显著提升了成像速度。光片显微镜问世以来，其在生命科学研究中的应用范围逐渐拓宽，从胚胎学、神经科学到肿瘤研究等多个领域均有所涉及，不仅可用于观察细胞和组织的基本结构，还可用于实时监测生物过程中的动态变化。同时，其跨尺度的特点使其适用于从宏观到微观的多个尺度上的观察。本文综述了光片显微镜在高通量成像、超分辨成像以及易用性方面的应用及发展，旨在为生命科学研究人员提供全面的了解和参考，推动光片显微镜在更多领域的应用和发展。

In recent decades, the advent of light sheet fluorescence microscopy as an innovative technique in fluorescence microscopy has significantly enhanced the high spatiotemporal resolution imaging capabilities of tissue and cellular structures and functions in life science research. Compared to traditional epi-fluorescence microscopy techniques, light sheet microscopy illuminates biological samples selectively, greatly improving photon utilization efficiency, reducing phototoxicity, and significantly increasing imaging speed. Since its introduction, light sheet microscopy has gradually expanded its application field in life science research, ranging from embryology and neuroscience to tumor studies, among others. It can not only be used to observe the basic structures of cells and tissues but also for real-time monitoring of dynamic changes in biological processes. Furthermore, its multiscale characteristics make it suitable for observations across multiple scales from macro to micro. This article reviews the applications and developments of light sheet microscopy in high-throughput imaging, high-precision imaging, and usability, aiming to provide life science researchers with comprehensive understanding and reference, and to promote its application and development in more fields.