中国光学, 2019, 12 (1): 104, 网络出版: 2019-03-06   

多光子皮肤成像技术及其应用

Multi-photon skin tissue imaging technology and its applications
作者单位
1 北京大学 工学院 生物医学工程系, 北京 100871
2 北京大学 元培学院, 北京 100871
摘要
多光子成像技术是一种层析能力好、信噪比高的新型光学成像技术。在皮肤光学三维检测中, 多光子技术已经应用于无创在体成像, 且已得到产业化开发。本文将首先介绍多光子皮肤检测系统的若干核心技术, 即双光子自发荧光技术、二次谐波成像技术、荧光寿命成像技术、相干反斯托克斯-拉曼成像技术等, 然后简要介绍多光子成像系统在皮肤疾病成像检测上的应用, 最后分析该系统的优势和未来可能的发展趋势。
Abstract
Multi-photon imaging is a new technique for optical imaging with inherent optical sectioning abilities and a high signal-to-noise ratio. Multi-photon microscopy has applications in dermatological imaging for its non-invasive and in situ imaging capability. As such, it has been applied commercially. In this paper, we first introduce the core technologies of multiphoton microscopy including two-photon auto-fluorescence imaging, second-harmonic generation(SHG), fluorescence life time imaging(FLIM) and coherent anti-Stokes Raman scattering(CARS). We then introduce its application on skin imaging analysis. Finally, we analyze the advantages and the possible future development of this technology.
参考文献

[1] KONIG K,RIEMANN I. High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution[J]. J. Biomed Opt.,2003,8(3):432-439.

[2] ZHANG Y,HONG H,CAI W. Photoacoustic imaging[J]. Cold Spring Harb Protoc,2011,2011(9):1015-1025.

[3] HUANG D,SWANSON E A,LIN C P,et al.. Optical coherence tomography[J]. Science,1991,254(5035):1178-1181.

[4] DENK W,STRICKLER J H,WEBB W W. Two-photon laser scanning fluorescence microscopy[J]. Science,1990,248(4951):73-76.

[5] KNIG K. Multiphoton Tomography[OL].[2018-02-20].http:www.mpt-tomography.de.

[6] KNIG K. Clinical multiphoton tomography[J]. J. Biophotonics,2008,1(1):13-23.

[7] BREUNIG H,WEINIGEL M,BCKLE R,et al.. Clinical coherent anti-Stokes Raman scattering and multiphoton tomography of human skin with a femtosecond laser and photonic crystal fiber[J]. Laser Physics Letters,2013,10(2):025604.

[8] MONICI M. Cell and tissue autofluorescence research and diagnostic applications[J]. Biotechnol Annu Rev,2005,11:227-256.

[9] 席鹏,刘宇嘉,姚志荣,等.用于皮肤影像诊断的光学成像方法[J].中国激光,2011,38(2):7-19.

    XI P,LIU Y J,YAO ZH R,et al.. Optical imaging techniques in skin imaging diagnosis[J]. Chinese Journal of Lasers,2011,38(2):7-19.(in Chinese)

[10] JAMME F,KASCAKOVA S,VILLETTE S,et al.. Deep UV autofluorescence microscopy for cell biology and tissue histology[J]. Biol. Cell,2013,105(7):277-288.

[11] SEIDENARI S,ARGINELLI F,BASSOLI S,et al.. Multiphoton laser microscopy and fluorescence lifetime imaging for the evaluation of the skin[J]. Dermatol Res Pract,2012,2012:810749.

[12] GIBSON E A,MASIHZADEH O,LEI T C,et al.. Multiphoton microscopy for ophthalmic imaging[J]. Journal of Ophthalmology,2011,2011:870879.

[13] BALU M,ZACHARY C B,HARRIS R M,et al.. In Vivo multiphoton microscopy of basal cell carcinoma[J]. JAMA Dermatol,2015,151(10):1068-1074.

[14] TSAI T H,JEE S H,DONG C Y,et al.. Multiphoton microscopy in dermatological imaging[J]. J. Dermatol Sci.,2009,56(1):1-8.

[15] KNIG K. Fluorescence Lifetime Imaging[OL].[2018-02-20].http://mpt-tomography.de/fluorescence-lifetime-imaging.

[16] DANCIK Y,FAVRE A,LOY C.J,et al.. Use of multiphoton tomography and fluorescence lifetime imaging to investigate skin pigmentation in vivo[J]. J. Biomed Opt.,2013,18(2):26022.

[17] WEINIGEL M,BREUNIG H G,KELLNER-HFER M,et al.. In vivo histology: optical biopsies with chemical contrast using clinical multiphoton/coherent anti-Stokes Raman scattering tomography[J]. Laser Physics Letters,2014,11(5):055601.

[18] KNIG K. In-Vivo Clinical Applications[OL].[2018-02-20]. http://mpt-tomography.de/vivo-clinical-applications.

[19] KOEHLER M J,HAHN S,PRELLER A,et al.. Morphological skin ageing criteria by multiphoton laser scanning tomography:non-invasive in vivo scoring of the dermal fibre network[J]. Exp. Dermatol,2008,17(6):519-523.

[20] LIN S J,WU R,J R,TAN H Y,et al.. Evaluating cutaneous photoaging by use of multiphoton fluorescence and second-harmonic generation microscopy[J]. Opt. Lett.,2005,30(17):2275-2277.

[21] SONG W,XU Q,ZHANG Y,et al.. Fully integrated reflection-mode photoacoustic, two-photon, and second harmonic generation microscopy in vivo[J]. Sci. Rep.,2016,6:32240.

应亚宸, 张广杰, 贾荟琳, 陆政元, 石玉洁, 席鹏. 多光子皮肤成像技术及其应用[J]. 中国光学, 2019, 12(1): 104. YING Ya-chen, ZHANG Guang-jie, JIA Hui-lin, LU Zheng-yuan, SHI Yu-jie, XI Peng. Multi-photon skin tissue imaging technology and its applications[J]. Chinese Optics, 2019, 12(1): 104.

本文已被 3 篇论文引用
被引统计数据来源于中国光学期刊网
引用该论文: TXT   |   EndNote

相关论文

加载中...

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!