研究了一种基于激光干涉技术的瞬态热力学量测量方法,可用于测量由于光激发产生的瞬时反应热及反应体积。介绍了利用干涉技术测量化学反应热量及反应体积的基本原理。实验中以肌红蛋白(Mb)为研究对象,由于肌红蛋白受光激发后,会与CO发生化学反应,导致系统的热力学量发生变化,从而引起系统折射率的变化。折射率变化会引起干涉相位变化。通过测量相位变化,可以获得肌红蛋白受光激发后热力学量的变化信息。在273～301 K温度范围内进行了肌红蛋白与CO的反应实验,测量了肌红蛋白与CO在10-6～10-1 s窗口的化学反应。测得的反应热量与体积分别为80 kJ/mol和10 mL/mol。

A micro calorimetry based on interferometer to measure the light-induced reaction volume and quantity of heat is studied. The principle to get the reaction volume and quantity of heat is introduced. The method is used to investigate the reaction of myoglobin (Mb) with CO after light excitation. Accompanying the reaction of Mb with CO, the quantity of heat and reaction volume are changed which could lead to the variation of the index of refraction of Mb. The thermodynamic information could be obtained through measuring the phase variation. The experiment to get the reaction volume and quantity of heat for the reaction of Mb with CO is done at 273 K to 301 K with a time window of 10-6 s to 10-1 s. The measured reaction enthalpy is 80 kJ/mol, the reaction volume is 10 mL/mol.