发光学报, 2011, 32 (10): 1088, 网络出版: 2011-10-21   

心肌梗死患者红细胞的光镊拉曼光谱

Red Blood Cells in Patients with Myocardial Infarction Using Optical Tweezers Raman Spectroscopy
作者单位
广西医科大学 物理学教研室, 广西 南宁 530021
摘要
利用激光光镊拉曼光谱系统, 测定了健康者红细胞和心肌梗死患者的红细胞拉曼光谱, 研究其拉曼光谱之间存在的差异性, 探讨其红细胞内容物的改变情况及其变化机理, 为疾病的诊断提供一种新的、快速简便的光谱分析手段, 为临床诊断提供有力的实验依据。
Abstract
Making use of optical tweezers Raman spectroscopy system to study the differences between normal human red blood cells and the red blood cell RBC in patients with myocardial infarction. We also investigate the changes of the contents in RBC and its variation mechanism. It is hopeful to provide a new, quick and easy method to diagnosis this disease, and may provide reliable experimental evidence for clinical diagnosis.
参考文献

[1] Xie C A, Dinno M A, Li Y Q. Near-infrared Raman spectroscopy of single optically trapped biological cells[J]. Opt. Lett., 2002, 27(4):249-251.

[2] Chottard G, Kazanskaya I, Bruschi M. Resonance Raman study of multihemic c-type cytochromes from desulfuromonas acetoxidans [J]. Eur. J. Biochem., 2000, 267(4):1050-1058.

[3] Tang H Y, Yao H L, Wang G W, et al. NIR Raman spectroscopic investigation of single mitochondria trapped by optical tweezers [J]. Opt. Express, 2007, 15(20):12708-12716.

[4] Wood B R, Caspers P, Puppels G J, et al. Resonance Raman spectroscopy of red blood cells using near-infrared laser excitation [J].Anal. Bioanal. Chem., 2007, 387(5):1691-1703.

[5] Lai J Z , Wang G W, Zhao C G, et al. Analysis of the Raman spectrum of single tricholoma lobayense spores trapped by laser tweezers [J]. Chin. J. Spectroscopy Laboratory (光谱实验室), 2010, 27(2):545-549 (in Chinese).

[6] Yue L Y, Wang G W, Yao H L, et al. Study of Raman spectroscopy of single human red blood cell trapped optically after thawed [J]. Acta Biophys Sin. (生物物理学报), 2007, 23(2):75-80 (in Chinese).

[7] Ai M, Liu J X, Yao H L. Raman spectra of single reticulocytes and small lymphocytes in blood[J]. Acta Optica Sinica (光学学报), 2009, 29(4):1-6 (in Chinese).

[8] Xie C A, Chen De, Li Y Q. Raman sorting and identification of single living micro-organisms with optical tweezers [J]. Opt. Lett., 2005, 30(14):1800-1802.

[9] Xie C A, Li Y Q. Co focal micro-Raman spectroscopy of single biological cell using optical trapping and shifted excitation deference techniques [J]. Appl. Phys., 2003, 93(5):2982-2986.

[10] Yang F Y. Recent advances in the study of membrane structure[J]. Progress In Biochemistry and Biophysics (生物化学与生物物理进展), 2003, 30(4):495-502 (in Chinese).

[11] Chen Y L, Cheng Y. Laser Raman spectroscopic study on membranous fluidity of human erythrocyte[J]. J. Medical Physics (中华物理学杂志), 1998, 8(11):169-171 (in Chinese).

[12] Carey P R. Biochemical Applications of Raman and Resonance Raman Spectroscopes [M]. New York: Academic Press, 1982:71-98.

[13] Tu A T. Raman Spectroscopy in Biology: Principles and Applications [M]. New York: Wiley, 1993:41-98.

[14] Chen X L , Wang G W, Liu J X, et al. Technique and advance of raman spectroscopy on functional erythrocyte study [J]. Acta Laser Biology Sinica (激光生物学报), 2008, 17(6):845-852 (in Chinese).

[15] Wang M, Yu F, Long Q. The application and development of Raman spectroscopy on protein conformation[J]. Acta Laser Biology Sinica (激光生物学报), 2007, 16(4):516-520 (in Chinese).

[16] Yang J, Yang T S. Relationship between the erythrocyte deformability and erythrocyte membrane lipid per oxidation injury and plasma sC5b9 in patients with acute myocardial infarction [J]. Medical Journal of QILU (齐鲁医学杂志), 2002, 17(2):119-120 (in Chinese).

吴智辉, 黄代政, 陈朝旺, 莫华. 心肌梗死患者红细胞的光镊拉曼光谱[J]. 发光学报, 2011, 32(10): 1088. WU Zhi-hui, HUANG Dai-zheng, CHEN Chao-wang, MO Hua. Red Blood Cells in Patients with Myocardial Infarction Using Optical Tweezers Raman Spectroscopy[J]. Chinese Journal of Luminescence, 2011, 32(10): 1088.

本文已被 1 篇论文引用
被引统计数据来源于中国光学期刊网
引用该论文: TXT   |   EndNote

相关论文

加载中...

关于本站 Cookie 的使用提示

中国光学期刊网使用基于 cookie 的技术来更好地为您提供各项服务,点击此处了解我们的隐私策略。 如您需继续使用本网站,请您授权我们使用本地 cookie 来保存部分信息。
全站搜索
您最值得信赖的光电行业旗舰网络服务平台!