应用光电阴极探测灵敏度为0.5 cps/mm2的超高灵敏度的光电成像系统,获得了绿豆芽和活体昆明鼠的超微弱生物发光图像,并用统计理论研究了极弱光强条件下光子图像的信号检验问题。文中在信号和噪声均为泊松分布的条件下,分析了从光子噪声中检验是否有信号的判据以及影响到检验的5个因素对检验结论的影响。以此判据成功地检验到实验获得的昆明鼠发光光子图像中的信号。

An ultra-sensitive imaging system with photocathode sensitivity of 0. 5 cps/mm2 has been developed to obtain the ultra-weak bioluminescence images of a sprounting Mung bean and a living Kunming mouse, and analysis with statistical theory was given for signal testing in these ultra-weak images. In this paper we analyzed the statistical judge to decide whether or not there is signal burying in noise under the condition that both signal and noise obey Poisson probability law, and discussed the five factors which have effects on the judge. With this judge, we have succeed in finding signal in the image of ultra-weak bioluminescence emitted by a living Kunming mouse.