DIC imaging for identification of motor and sensory nerves

Dayu Chen; Yuxiang Wu; Tao Sui; Shaoqun Zeng; Xiaojian Cao; Xiaohua Lv

doi:doi:10.1142/s179354581643001x

Journal of Innovative Optical Health Sciences, 2016, 9 (5): 1643001, Published Online: Dec. 27, 2018

DIC imaging for identification of motor and sensory nerves

论文大纲

Dayu Chen ¹Yuxiang Wu ^2,3Tao Sui ⁴Shaoqun Zeng ²Xiaojian Cao ^4,*Xiaohua Lv ²

Author Affiliations

¹ Department of Neurosurgery Wuhan General Hospital of Guangzhou Military Command of PLA 627 Wuluo Road, Wuhan 430070, P. R.China

² Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074, P. R. China

³ School of Physical Education, Jianghan University Wuhan 430056, P. R. China

⁴ First Affiliated Hospital of Nanjing Medical University Nanjing 210029, P. R. China

Differential interference microscopy nerve repair nerve root image pattern

Abstract

Identification of motor and sensory nerves is important in applications such as nerve injury repair. Conventional practice relies on time consuming staining methods for this purpose. Here, we use laser scanning infrared differential interference contrast (IR-DIC) microscopy for label-free observation of the two types of nerve. Ventral and dorsal nerve roots of adult beagle dogs were collected and sections of different thicknesses were imaged with an IR-DIC microscope. Different texture patterns of the IR-DIC images of the motor and sensory nerve can be distinguished when the section thickness increases to 40 μm. This suggests that nerve fibers in motor and sensory nerves have different distribution patterns. The result hints a potential new way for more rapid identification of nerve type in peripheral nerve repair surgery.

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Dayu Chen, Yuxiang Wu, Tao Sui, Shaoqun Zeng, Xiaojian Cao, Xiaohua Lv. DIC imaging for identification of motor and sensory nerves[J]. Journal of Innovative Optical Health Sciences, 2016, 9(5): 1643001.

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