光子学报, 2017, 46 (6): 0612004, 网络出版: 2017-06-27  

毛细管电泳噪声特性分析

Analysis of Capillary Electrophoresis Noise Characteristics
作者单位
1 常州工学院 电气与光电工程学院, 江苏 常州 213022
2 华东理工大学 理学院, 上海 200237
摘要
为优化毛细管电泳荧光信号检测系统,提高检测灵敏度,以100~1 000碱基对的脱氧核糖核酸作为分离对象,羟乙基纤维素为筛分介质,研究了直流电场下毛细管电泳荧光信号检测系统中的噪声特性.对不同分离电场强度、羟乙基纤维素溶液浓度和分子量、毛细管有效长度以及毛细管内径形状等情况下的噪声特性进行分析.分析得到该检测系统中信噪比最佳的优化参量,即分离电场强度为500~600 V/cm、羟乙基纤维素浓度为0.6%~0.7%、羟乙基纤维素分子量为250、圆形内径为50 μm以及毛细管有效长度为8 cm.
Abstract
In order to optimize the fluorescence detection capillary electrophoresis system and improve the detection sensitivity, the signal noise characters were studied when separating 100~1 000 base pair deoxyribonucleic acid ladder under direct current electric field in hydroxyethyl cellulose solutions. The dependence of signal noise on separation electric field intensity, polymer concentration, hydroxyethyl cellulose molecular weight, the effective length and the capillary shape were investigated. Experiment result shows that optimum parameters can be obtained under best signal noise ratio, which are the separation electric field strength of 500~600 V/cm, hydroxyethyl cellulose concentration of 0.6%~0.7%、hydroxyethyl cellulose molecular weight of 250, round inner diameter of 50 μm and effective capillary length of 8 cm.
参考文献

[1] CHENG Hui-ling, CHIOU Shyh Shin, LIAO Yu-mei, et al. Genotyping of single nucleotide polymorphism in γ-glutamyl hydrolase gene by capillary electrophoresis[J]. Electrophoresis, 2011, 15: 2021-2027.

[2] 刘圆圆,王荣,贾正平,等.毛细管电泳-限制性片段长度多态性分析检测胃癌H-ras基因点突变[J].化学学报, 2009,67(4): 323-328.

    LIU Yuan-yuan, WANG Rong, JIA Zheng-ping, et al. H-ras gene mutation detection of gastric cancer by restriction fragment length polymorphism with capillary electrophoresis[J]. Acta Chimica Sinica, 2009, 67(4): 323-328.

[3] 奚正山, 杨瑛, 马文丽,等. 小波去噪与信号相似性在电泳信号方面的应用[J]. 微电子技术, 2004, 24: 24-27.

    XI Zheng-shan, YANG Yong, MA Wen-li, et al. Application of wavelet denoising and signal resemble quality in electrophoresis signal[J]. Microelectronic Technology, 2004, 24: 24-27.

[4] 史大明, 瞿海斌, 程翼宇. 基于在线小波变换的毛细管电泳信号滤噪方法[J]. 江南大学学报(自然科学版), 2007, 6(3): 340-344.

    SHI Da-ming, QU Hai-bin, CHENG Yi-yu. Filter of capillary electrophoresis signals based on online wavelet transform[J]. Journal of Jiangnan University (Natural Science Edition), 2007, 6(3): 340-344.

[5] 郑华, 石岩, 汪洁,等. DNA测序电泳荧光信号的小波去噪分析[J]. 光学仪器, 2007, 29(2): 17-21.

    ZHEN Hua, SHI Yan, WANG Jie, et al. Analyzing the wavelet denoising of electrophoresis fluoreseenee signals for DNA sequencing[J]. Optical Instruments, 2007, 29(2): 17-21.

[6] 沈鑫, 杜宇人. 一种基于最优小波包基的电泳荧光信号去噪方法[J]. 扬州大学学报(自然科学版), 2011, 14(2): 70-73.

    SHEN Xin, DU Yu-ren, WANG Jie, et al. A novel denoising algorithm for electrophoretic fluorescent signal based on the best basis of wavelet packets[J]. Journal of Yangzhou University (Natural Science Edition), 2011, 14(2): 70-73.

[7] 石岩, 王立强, 郑华,等. 激光诱导荧光毛细管电泳DNA检测系统信噪比分析[J]. 光子学报, 2008, 37(7): 1446-1449.

    SHI Yan, WANG Li-qiang, ZHENG Hua, et al. Signal to noise ratio analysis for DNA detecting system by capillary electrophoresis and laser-induced fluorescence[J]. Acta Photonica Sinica, 2008, 37(7): 1446-1449.

[8] YU L, LACEY M E, SWEEDLER O V, et al. Spectral restoration from low signal-to-noise, distorted NMR signals application to hyphenated capillary electrophoresis-NMR[J]. Journal of Magnetic Resonance, 2003, 162: 133-140.

[9] EMY G L, CALISTO V, ESTEVES V I. Noise normalisation in capillary electrophoresis using a diode array detector[J]. Journal of Separation Science, 2011, 34: 1703-1707.

[10] 原育凯. 光学系统杂散光的消除方法[J]. 大气与环境光学学报, 2007, 2(1): 6-10.

    YUAN Yu-kai. Stray light suppressing measure of optieal system[J]. Journal of Atmospheric and Environment Optics, 2007, 2(1): 6-10.

[11] 何峥嵘. 运算放大器电路的噪声分析和设计[J]. 微电子学, 2006, 36(2): 148-153.

    HE Zheng-rong. Noise analysis and design of operational amplifier circuits[J]. Microelectronics, 2006, 36(2): 148-153.

[12] 叶莉华, 汪海洋, 王文轩,等. 基于光电倍增管的低噪声前置放大器的设计及其信号处理[J].电子器件, 2013, 36(3): 340-343.

    YE Li-hua, WANG Hai-yang, WANG Wen-xuan, et al. Design of low- noise preamplifier based on PMT and its signal processing[J]. Chinese Journal of Electron Devices, 2013, 36(3): 340-343.

[13] KIM H, CHEN C T, RONZHIN A, et al. A silicon photo-multiplier signal readout using strip-line and waveform sampling for positron emission tomography[J]. Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 2016, 830: 119-129.

[14] 周荣楣. 光电发射、次级电子发射与光电倍增管[M]. 成都: 电子科技大学出版社, 2015.

[15] 王文祥. 真空电子器件[M]. 北京: 国防工业出版社, 2012.

[16] 刘晨晨, 李振庆, 孟凡明, 等. 羟乙基纤维素中毛细管电泳DNA分离特性研究[J]. 化学学报, 2013, 71: 265-270.

    LIU Chen-chen, LI Zhen-qing, MENG Fan-ming, et al. Capillary electrophoresis of DNA in Hydroxyethylcellulose[J]. Acta Chimica Sinica, 2013, 71: 265-270.

陈功, 朱锡芳, 窦晓鸣, 刘晨晨, 许清泉, 山口佳则, 倪一. 毛细管电泳噪声特性分析[J]. 光子学报, 2017, 46(6): 0612004. CHEN Gong, ZHU Xi-fang, DOU Xiao-ming, LIU Chen-chen, XU Qing-quan, YAMAGUCHI Yoshinori, NI Yi. Analysis of Capillary Electrophoresis Noise Characteristics[J]. ACTA PHOTONICA SINICA, 2017, 46(6): 0612004.

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