采用氧合血红蛋白吸收带的漫反射光谱比率R540/R575检测人胃癌

采用氧合血红蛋白吸收带的平均漫反射光谱比率(R540/R575)对离体的人正常胃、未分化胃腺癌、胃鳞状细胞癌和低分化胃腺癌的上皮粘膜组织进行鉴别诊断。实验采用带积分球附件的分光光度计测量组织样品在400 nm-600 nm的漫反射光谱。实验结果表明,在相同的波长范围内,人的未分化胃腺癌、胃鳞状细胞癌和低分化胃腺癌的上皮粘膜组织的漫反射光的强度较人正常胃的上皮粘膜组织的漫反射光强度要显著地低的,尤其是在氧合血红蛋白的吸收带540 nm和575 nm波长处,人正常胃、未分化胃腺癌、胃鳞状细胞癌和低分化胃腺癌的上皮粘膜组织的平均漫反射光谱比率分别是(94.2±2.4)%,(109±3.0)%,(103±2.8)%和(98.6±2.6)%。

Abstract

Differential diagnosis of epithelial tissues of normal human gastric,undifferentiated gastric adenocarcinoma,gastric squamous cell carcinomas,and poorly differentiated gastric adenocarcinoma were studied using the mean diffuse reflectance spectral ratio (R540/R575) of the oxygenated hemoglobin absorption bands. Exvivo diffuse reflectance spectra in the 400 nm to 600nm region was measured from surgically removed gastric lesions using a spectrophotometer with an integrating sphere attachment. The results showed that the reflectance spectral intensity of the malignant tissues was lower than that of human normal gastric tissues and showed dips at 540 nm and 575 nm owing to absorption from oxygenated hemoglobin. Measurements carried out,the diffuse reflectance spectral ratio for epithelial tissues of normal human gastric,undifferentiated gastric adenocarcinoma,gastric squamous cell carcinomas,and poorly differentiated gastric adenocarcinoma were (94.2±2.4)%,(109±3.0)%,(103±2.8)% and (98.6±2.6)% respectively.

参考文献

[1] Li Xiaozhou,Lin Junxiu,Jia Chunde et al.. Deterction and charaterization of stomach cancer and atrophic gastric with fluorescence and raman spectroscopy[C]. SPIE,2003,5255:93-98

[2] 师英强. 关于早期胃癌临床诊断的若干问题[J]. 外科理论与实践,2008,13(1):7-8

Shi Yingqiang. Some problems in clinical examination of early gastric carcinoma[J]. J. Surgery Concepts& Practice,2008,13(1):7-8

[3] Katja Wirths,Horst Neuhaus. Endomicroscopy of gatritis and gastric cancer[J]. SpringerLink Endomicroscopy of GI Disorders,2007,7:60-66

[4] 李延青. 共聚焦内镜在胃肠道肿瘤早期诊断中的应用[J]. 中国实用内科杂志,2008,28(3):238-240

Li Yanqing. Confocal endoscopy for early diagnosing gastrointestinal neoplasias[J]. Chinese J. Pratical Intemal Medicine,2008,28(3):238-240

[5] M.L. Fraser Monteiro,T. Rezio,Jorge Soares et al.. Flourescence spectroscopy of normal and cancerous human stomach tissue[C]. SPIE,1993,2081:230-236

[6] Melissa C. Skala,Gregory M. Palmer,Kristin M. Vrotsos et al.. Comparision of a physical model nd principal component analysis for the diagnosis of epithelial neoplasias in vivo using diffuse reflectance spectroscopy[J]. Opt. Express,2007,15(12):7863-7875

[7] Jarod C. Finlay,Timothy C. Zhu,Andreea Dimofte et al.. Diffuse reflectance spectra measured in vivo in human tissues during photofrin-mediated pleural photodynamic therapy[C]. SPIE,2006,6139:613900-1

[8] Li Xiaozhou,Lin Junxiu. Gastroentric cancers detection by raman spectroscopy of human serum[C]. SPIE,2003,4952:162-168

[9] H. Barr,T. Dix,N.Stone. Optical spectroscopy for the early diagnosis of gastrointestinal malignancy[J]. Lasers Med. Sci.,1998,13(1):3-13

[10] D. W. Lee,K. W. Seo,B. R. Min. Discrimination between cancer and normal tissue using near infrared spectroscopy[J]. MBEC,2008,2(08):1341-1344

[11] 王秀琳,李步洪,谢树森. 组织体漫反射率与光学特性参量关系的研究[J]. 光电子·激光,2001,12(4):417-420

Wang Xiulin,Li Buhong,Xie Shunsen. Experimental studies of diffuse reflectance vs optical properties of Biotissue[J]. J. Optoelectronics Laser,2001,12(4):417-420

[12] 谢树森,郑蔚,李步洪等. 紫激光辐射人肺组织的光学特性[J]. 光学学报,2000,20(2):229-233

Xie Shusun,Zheng Wei,Li Buhong et al.. Optical properties of normal and cancerous human lung tissues irradiated by a violet Kr+ laser[J]. Acta Optica Sinica,2000,20(2):229-233

[13] 魏华江,邢达,巫国勇等. 采用空间分辨漫反射测定胃组织光学特性[J]. 中国激光,2007,34(4):582-587

Wei Huajiang,Xing Da,Wu Guoyong et al.. Using spatially resolved reflectance to measure optical properties of stomach tissue[J]. Chinese J. Lasers,2007,34(4):582-587

[14] 朱丹,骆清铭,曾绍群等. 热作用下蛋白及全血光学特性变化的实验研究[J]. 光学学报,2002,22(3):369-373

Zhu Dan,Luo Qingming,Zeng Shaoqun et al.. Change in the optical properties of slowly heated human whole blood and albumen[J]. Acta Optica Sinica,2002,22(3):369-373

[15] Melissa C. Skala,Gregory M. Palmer,Changfang Zhu et al.. Investigation of fiber-optical probe designs for optical spectroscopic dianosis of epithelialpre-cancers[J]. Lasers in Surgery and Medicine,2004,34(1):25-38

[16] N. Subhash,J. R. Mallia,S. S. Thomas et al.. Oral cancer detection using diffuse reflectance spectral ratio R540/R575 of oxygenated hemoglobin bands[J]. J. Biomed. Opt.,2006,11(1):014018-1

[17] M. Hammer,D. Schweitzer. Quantitative reflection spectroscopy at the human ocular fundus[J]. Phys. Med. Biol,2002,47(2):179-191

[18] R. K. Jain. Determinants of tumor blood flow:a review[J]. Cancer Res.,1988,48(10):2641-2658

[19] Alexander A. Stratonnikow,Victor B. Loschenov. Evaluation of blood oxygen saturation in vivo from diffuse reflectance spectra[J]. J. Biomed.Opt.,2001,6(4):457-567

[20] N. M. Kuzin,V. B. Loschenov,A. A. Stratonnikov et al.. Method of oxygenation saturation evaluation of stomach mucous after subtotal cancer resection[C]. SPIE,1994,2328:98-101

[21] Yuanlong Yang,Edward J. Celmer,Jason A .Koutcher et al.. DNA and protein changes caused by disease in human breast tissues probed by the kubelka-munk spectral function[J]. Photochemistry and Photobiology,2002,75(6):627-632

[22] 王成,范瑾,任秋实. 癌变组织可见光反射光谱测量[J]. 光谱学与光谱分析,2008,28(1):33-36

Wang Cheng,Fan Jin,Ren Qiushi. Visible light reflectance spectrum for measurement of cancerous tissue[J]. Spectroscopy and Spectral Analysis,2008,28(1):33-36

[23] W. G. Zijistra,A. Buursma,W. P. Meeuwsen-Vander Roest. Absorption specrea of human fetal and adult oxyhemoglobin,de-oxyhemoglobin carboxyhemoglobin,and Methemoglobin[J]. Clin. Chem.,1991,37(9):1633-1638

李兰权, 魏华江, 何博华, 郭周义, 杨洪钦, 谢树森, 陈雪梅, 李力波, 巫国勇, 鲁建军. 采用氧合血红蛋白吸收带的漫反射光谱比率R540/R575检测人胃癌[J]. 光学学报, 2009, 29(10): 2860. Li Lanquan, Wei Huajiang, He Bohua, Guo Zhouyi, Yang Hongqin, Xie Shusen, Chen Xuemei, Li Libo, Wu Guoyong, Lu Jianjun. Gastric Cancer Detection Using Diffuse Reflectance Spectral Ratio R540/R575 of Oxygenated Hemoglobin Bands[J]. Acta Optica Sinica, 2009, 29(10): 2860.

采用氧合血红蛋白吸收带的漫反射光谱比率R540/R575检测人胃癌

关于本站 Cookie 的使用提示

全站搜索

采用氧合血红蛋白吸收带的漫反射光谱比率R540/R575检测人胃癌

相关论文

相关资讯

关于本站 Cookie 的使用提示

全站搜索