一种基于共轴干涉的相位物体定量成像技术

吴舒哲; 唐嘉; 熊亮; 黄佐华

doi:doi:10.7510/jgjs.issn.1001-3806.2017.02.026

激光技术, 2017, 41 (2): 275, 网络出版: 2017-03-29

一种基于共轴干涉的相位物体定量成像技术

A technique of quantitative imaging for phase object based on in-line interfermetry

论文大纲

吴舒哲唐嘉熊亮黄佐华 ^*

作者单位

华南师范大学物理与电信工程学院量子调控工程与材料实验室, 广州 510006

成像系统定量相位成像干涉相位物体单幅或两幅相衬图 imaging systems quantitative phase imaging interference phase objects one or two phase contrast images

摘要

为了实现生物组织及相位光栅等透明相位物体的快速定量相位测量与成像, 基于共轴干涉及相衬干涉原理, 构建了一套相位物体定量相位快速测量与成像系统。分析了定量成像原理, 设计了相应的相位提取及恢复算法。通过拍摄单幅或两幅相衬图, 实现了相位光栅、水滴等小相位变化的相位样品的定量相位测量与成像。设计了相应的分区差分相位解包裹算法, 实现了相位值超过π的微透镜的定量相位测量与成像。结果表明, 通过该系统测量所得到的全息相位光栅的相位分布以及不同超声驱动电压下弱超声驻波光栅的相位振幅变化关系与其它方法所得的测量结果基本一致; 微透镜的实验测量厚度值与理论计算值相比, 绝对误差约为0.03μm。本系统具有一定的可行性和适应性, 在生物细胞和组织等透明相位物体的快速测量与成像方面有潜在的应用意义。

Abstract

In order to implement rapid quantitative phase measurement and imaging for transparent biological tissues, phase grating and other phase objects, based on the principle of in-line interferometry and phase contrast interferometry, an experimental system for fast quantitative measurement and imaging of phase objects was established. The principle of quantitative imaging was analyzed and the corresponding phase extraction and recovery procedures were designed. By shooting one or two phase contrast interferograms, the quantitative phase imagings for phase grating, water microdroplet and other phase objects with small phase variation were realized by the proposed method. A corresponding partition differential phase unwrapping algorithm was designed and the quantitative phase measurement and imaging of micro lens with phase value over π was achieved. The results show that the relationship between phase distribution of the obtained holographic phase grating measured by the system and phase amplitude variation of weak ultrasonic standing wave grating under different ultrasonic driving voltages is basically consistent with the results obtained from other methods. The experimental measurement of micro lens is compared with the theoretical value, and the absolute error is about 0.03μm. This system has certain feasibility, adaptability and potential applications on fast measurement and imaging of transparent phase objects such as biological cells and tissues.

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吴舒哲, 唐嘉, 熊亮, 黄佐华. 一种基于共轴干涉的相位物体定量成像技术[J]. 激光技术, 2017, 41(2): 275. WU Shuzhe, TANG Jia, XIONG Liang, HUANG Zuohua. A technique of quantitative imaging for phase object based on in-line interfermetry[J]. Laser Technology, 2017, 41(2): 275.

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