Spectrophotometer with enhanced sensitivity for uric acid detection

Nurfatihah Che Abd Rashid; Nor Hafizah Ngajikin; Asrul Izam Azmi; Rashidah Arsat; Suhaila Isaak; Noran Azizan Cholan; Nur Ellina Azmi

doi:doi:10.3788/COL201917.081701

Chinese Optics Letters, 2019, 17 (8): 081701, Published Online: Jul. 26, 2019

Spectrophotometer with enhanced sensitivity for uric acid detection Download： 800次

Nurfatihah Che Abd Rashid ¹Nor Hafizah Ngajikin ^2,*Asrul Izam Azmi ¹Rashidah Arsat ¹Suhaila Isaak ¹Noran Azizan Cholan ²Nur Ellina Azmi ³

Author Affiliations

¹ Faculty of Electrical Engineering, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia

² Faculty of Electrical and Electronic Engineering, Universiti Tun Hussein Onn Malaysia, 86400 Bt. Pahat, Johor, Malaysia

³ Industrial Biotechnology Research Centre, SIRIM Berhad, 40200 Shah Alam, Selangor, Malaysia

Figures & Tables

Fig. 1. Configuration of spectrophotometer.

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Fig. 2. Samples with different uric acid concentrations (a) before and (b) after stirring.

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Fig. 3. Light beam at 10 mm distance from the input fiber. (a) Halogen beam. (b) Gray-scale percentage for halogen light. (c) LED beam (460 nm wavelength). (d) Gray-scale percentage for LED light.

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Fig. 4. Spectrometer output spectra. (a) Halogen. (b) LED (460 nm wavelength).

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Fig. 5. Light absorbance with variations of uric acid concentration at wavelengths of 460, 525, and 630 nm. (a) Halogen. (b) LED.

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Fig. 6. Human urine sample in cuvette after mixing with NaOH solution.

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Fig. 7. Absorbance analysis on human urine sample at 460 nm wavelength.

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Table1. Comparison of Light Characteristics Between Halogen and LED Light Sources at 460 nm Wavelength

Parameter	Halogen	LED
$P_{beam}$ (μW)	15.4	1.7
$A_{beam}$ ( ${mm}^{2}$ )	33.18	9.08
$φ_{interacted}$ (nlm)	7.3337	2.9582
$L_{e}$ (mlm/W)	0.4762	1.7401

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Table2. Comparison of Spectrophotometer Performance

Light Source	Wavelength (nm)	Sensitivity (dL/mg)	Accuracy (%)
Halogen	460	0.0012	86.51
	525	0.0011	91.17
	630	0.0008	84.27
LED	460	0.0046	96.62
	525	0.0031	77.57
	630	0.0045	79.43

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Nurfatihah Che Abd Rashid, Nor Hafizah Ngajikin, Asrul Izam Azmi, Rashidah Arsat, Suhaila Isaak, Noran Azizan Cholan, Nur Ellina Azmi. Spectrophotometer with enhanced sensitivity for uric acid detection[J]. Chinese Optics Letters, 2019, 17(8): 081701.

Spectrophotometer with enhanced sensitivity for uric acid detection Download： 800次

Fig. 1. Configuration of spectrophotometer.

Fig. 2. Samples with different uric acid concentrations (a) before and (b) after stirring.

Fig. 3. Light beam at 10 mm distance from the input fiber. (a) Halogen beam. (b) Gray-scale percentage for halogen light. (c) LED beam (460 nm wavelength). (d) Gray-scale percentage for LED light.

Fig. 4. Spectrometer output spectra. (a) Halogen. (b) LED (460 nm wavelength).

Fig. 5. Light absorbance with variations of uric acid concentration at wavelengths of 460, 525, and 630 nm. (a) Halogen. (b) LED.

Fig. 6. Human urine sample in cuvette after mixing with NaOH solution.

Fig. 7. Absorbance analysis on human urine sample at 460 nm wavelength.

Table1. Comparison of Light Characteristics Between Halogen and LED Light Sources at 460 nm Wavelength

Table2. Comparison of Spectrophotometer Performance

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Spectrophotometer with enhanced sensitivity for uric acid detection Download： 800次

Fig. 1. Configuration of spectrophotometer.

Fig. 2. Samples with different uric acid concentrations (a) before and (b) after stirring.

Fig. 3. Light beam at 10 mm distance from the input fiber. (a) Halogen beam. (b) Gray-scale percentage for halogen light. (c) LED beam (460 nm wavelength). (d) Gray-scale percentage for LED light.

Fig. 4. Spectrometer output spectra. (a) Halogen. (b) LED (460 nm wavelength).

Fig. 5. Light absorbance with variations of uric acid concentration at wavelengths of 460, 525, and 630 nm. (a) Halogen. (b) LED.

Fig. 6. Human urine sample in cuvette after mixing with NaOH solution.

Fig. 7. Absorbance analysis on human urine sample at 460 nm wavelength.

Table1. Comparison of Light Characteristics Between Halogen and LED Light Sources at 460 nm Wavelength

Table2. Comparison of Spectrophotometer Performance

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