数字全息显微系统的成像分辨率分析

根据成像理论和全息系统的点扩散函数,研究了数字全息显微系统的成像分辨率,给出了无预放大和有预放大情况下成像分辨率的表达式,指出了只有当CCD的成像分辨率等于或高于显微物镜(MO)的成像分辨率时,预放大数字全息系统的成像分辨率才取决于显微物镜的数值孔径(NA);反之,系统的成像分辨率则取决于CCD的数值孔径。采用无透镜傅里叶变换全息记录光路,对美国空军分辨率测试板进行了实验研究,结果表明再现像在水平和垂直方向的极限分辨率分别为3.91 μm和4.38 μm,与理论分析相吻合。对条纹物体进行了全息图的模拟记录和再现,结果与理论分析相一致。

Abstract

According to optical imaging theory and the point spread function of digital holographic system, the imaging resolution of the digital holographic microscopy was investigated. The imaging resolution expressions of the digital holographic systems with and without pre-magnification were presented in details. With those formulas, it is clearly shown that only when the imaging resolution of CCD is not below the resolution of the microscope objective (MO), the imaging resolution of the whole system is dependent on the numerical aperture (NA) of MO. Otherwise, it is dependent on the numerical aperture of the CCD. Experimental imaging of US air force-resolution test target is performed using the lensless Fourier transform configuration. The experimental results that ultimate resolution of reconstructed image in horizontal direction and vertical direction is 3.91 μm and 4.38 μm respectively. Finally, recording and reconstructing of holograms for stripe object are stimulated , and results are corresponding with theoretical analysis.

参考文献

[1] K. Volker, J. H. Hans, W. P. O. Jptner. Application of digital holographic microscopy for inspection of micro-optical components [C]. SPIE, 2001, 4398:189～198

[2] . Dirksen, H. Droste, B. Kemper et al.. Lensless Fourier holography for digital holographic interferometry on biological samples[J]. Opt. & Laser Eng., 2001, 26: 241-249.

[3] . Colomb, E. Cuche, F. Charriere et al.. Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation[J]. Appl. Opt., 2006, 45(5): 851-863.

[4] . Carl, B. Kemper, G. Wernicke et al.. Parameter-optimized digital holographic microscope for high-resolution living-cell analysis[J]. Appl. Opt., 2004, 43(36): 6536-6544.

[5] C. J. Mann, K. Kim. Quantitative phase-contrast microscopy by angular spectrum digital holography [C]. SPIE, 2006, 6090:60900B-1～60900B-8

[6] . Udupa, B. K. A. Ngoi, H. C. Freddy Goh et al.. Defect detection in unpolished Si wafers by shearography[J]. Measur. Sci. Tech., 2004, 15: 35-43.

[7] E. Novak. MEMS metrology techniques [C]. SPIE, 2005, 5716:173～181

[8] . Ferraro, S. Grilli, D. Alfieri et al.. Extended focused image in microscopy by digital holography[J]. Opt. Express, 2005, 13(18): 6738-6749.

[9] . C. Zhang, G. Pedrini, W. Osten. Reconstruction algorithm for high-numerical aperture holograms with diffraction-limited resolution[J]. Opt. Lett., 2006, 31(11): 1633-1635.

[10] Huaying Wang, Dayong Wang, Jianjun Xie et al.. Recording conditions of digital holography [C]. SPIE, 2007, 6279:62791J-1～62791J-10

[11] Fan Qi, Zhao Jianlin, Li Shiyang et al.. Detail displaying and vision aberration rectifying of reconstructed image in digital holography [J]. Chinese J. Lasers, 2005, 32(10):1401～1405
范琦,赵建林,李世扬等. 数字全息再现像的细节显示和视觉畸变矫正[J]. 中国激光, 2005, 32(10):1401～1405

[12] Zhong Liyun, Zhang Yimo, Lü Xiaoxu et al.. Some quantitative analysis about digital holographic reconstructed image [J]. Chinese J. Lasers, 2004, 31(5):570～574
钟丽云,张以谟,吕晓旭等. 数字全息图再现像的分析计算[J]. 中国激光, 2004, 31(5):570～574

[13] . . Imaging analysis of digital holographys[J]. Opt. Express, 2005, 13(7): 2444-2451.

[14] Fucai Zhang, G. Pedrini, W. Osten. Aberration free reconstruction algorithm for high numerical aperture digital hologram [C]. SPIE, 2006, 6188:618814-1～618814-8

[15] . . Effect of the fill factor of CCD pixels on digital holograms[J]. Opt. Eng., 2003, 42(9): 2768-2771.

[16] J. W. Goodman, Qin Kecheng, Liu Peisen, Chen Jiabi et al. trans.. Introduction to Fourier Optics [M]. Third edition. Beijing: Publishing House of Electronics Industry, 2006. 141～143
J. W. Goodman 著,秦克诚,刘培森,陈家璧等译. 傅里叶光学导论[M]. 第3版. 北京：电子工业出版社, 2006. 141～143

王华英, 王广俊, 赵洁, 谢建军, 王大勇. 数字全息显微系统的成像分辨率分析[J]. 中国激光, 2007, 34(12): 1670. 王华英, 王广俊, 赵洁, 谢建军, 王大勇. Imaging Resolution Analysis of Digital Holographic Microscopy[J]. Chinese Journal of Lasers, 2007, 34(12): 1670.

数字全息显微系统的成像分辨率分析

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