A SIMULTANEOUS MULTI-PROBE DETECTION LABEL-FREE OPTICAL-RESOLUTION PHOTOACOUSTIC MICROSCOPY TECHNIQUE BASED ON MICROCAVITY TRANSDUCER

YONGBO WU; ZHILIE TANG; YAN CHI; LIRU WU

doi:doi:10.1142/s1793545813500272

Journal of Innovative Optical Health Sciences, 2013, 6 (3): 1350027, Published Online: Jan. 10, 2019

A SIMULTANEOUS MULTI-PROBE DETECTION LABEL-FREE OPTICAL-RESOLUTION PHOTOACOUSTIC MICROSCOPY TECHNIQUE BASED ON MICROCAVITY TRANSDUCER

论文大纲

YONGBO WU ¹ZHILIE TANG ^1,2,*YAN CHI ¹LIRU WU ¹

Author Affiliations

¹ School of Physics and Telecom Engineering, South China Normal University Guangzhou 510006, P. R. China

² Laboratory of Quantum Information Technology, South China Normal University IMOT, Guangzhou 510006, P. R. China

Multi-probe label-free optical-resolution photoacoustic microscopy

Abstract

We demonstrate the feasibility of simultaneous multi-probe detection for an optical-resolution photoacoustic microscopy (OR-PAM) system. OR-PAM has elicited the attention of biomedical imaging researchers because of its optical absorption contrast and high spatial resolution with great imaging depth. OR-PAM allows label-free and noninvasive imaging by maximizing the optical absorption of endogenous biomolecules. However, given the inadequate absorption of some biomolecules, detection sensitivity at the same incident intensity requires improvement. In this study, a modulated continuous wave with power density less than 3mW/cm² (1/4 of the ANSI safety limit) excited the weak photoacoustic (PA) signals of biological cells. A microcavity transducer is developed based on the bulk modulus of gas five orders of magnitude lower than that of solid; air pressure variation is inversely proportional to cavity volume at the same temperature increase. Considering that a PA wave expands in various directions, detecting PA signals from different positions and adding them together can increase detection sensitivity and signal-to-noise ratio. Therefore, we employ four detectors to acquire tiny PA signals simultaneously. Experimental results show that the developed OR-PAM system allows the label-free imaging of cells with weak optical absorption.

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YONGBO WU, ZHILIE TANG, YAN CHI, LIRU WU. A SIMULTANEOUS MULTI-PROBE DETECTION LABEL-FREE OPTICAL-RESOLUTION PHOTOACOUSTIC MICROSCOPY TECHNIQUE BASED ON MICROCAVITY TRANSDUCER[J]. Journal of Innovative Optical Health Sciences, 2013, 6(3): 1350027.

A SIMULTANEOUS MULTI-PROBE DETECTION LABEL-FREE OPTICAL-RESOLUTION PHOTOACOUSTIC MICROSCOPY TECHNIQUE BASED ON MICROCAVITY TRANSDUCER

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